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Background: Paraquat, 1,1 '- dimethyl -4,4' - bipyridine dichloro-cation salt in the sixties of the last century found weeding role, it is rapidly inactivated after contact with the soil, atmospheric pollution and residual herbicidal effect is good, the price is cheap, Paraquat is widely used in the majority of agricultural countries. Each year due to the increase in accessibility, the high number of people interested in taking or wrongly. After paraquat ingestion, liver, kidney, heart, and even nerve tissue will be damaged, can lead to multiple organ dysfunction, in which the main target organ is the lung, can cause acute lung injury, advanced form of pulmonary fibrosis. Foreign literature in 1966 first reported two cases of the paraquat poisoning deaths, and then around the world have reported paraquat poisoning cases. Domestic nineties Zhongshan Medical University, first reported two cases of poisoning cases. The mechanism of paraquat-induced acute lung injury is not clear. Paraquat poisoning no specific antidote, the high mortality rate of acute poisoning. Therefore, the acute paraquat poisoning basic and clinical research is of great significance. Bosentan is a dual endothelin antagonist used in the treatment of pulmonary hypertension to improve exercise tolerance, improve the hemodynamics of patients with pulmonary hypertension, and to improve the quality of life of patients, there is evidence to show that its lung fibrosis treatment effect. There is currently no bosentan treatment of paraquat poisoning reported acute lung injury. Objective: To study the therapeutic effects of bosentan in paraquat-induced acute lung injury in animal models of. Methods: Adult male Wister rats 90, were randomly divided into three groups: the paraquat exposure group, the beginning of the experiment to give a one-time 20% paraquat dope orally 50mg/kg body weight of rats; paraquat bosentan treatment group, the start of the experiment a one-time allowance of 20% paraquat dope orally 50mg/kg body weight of rats, and then given daily bosentan 100mg/kg body weight of rats; blank control group was given normal saline orally. The rats were killed after the end of the experiment, respectively anesthesia 3,7,14,21 days before the animal stopped breathing venous blood remain seized. Enzyme-linked immunosorbent assay (ELISA) to detect serum TGF-β1 and endothelin levels. Sacrificing the rat lungs detection of transforming growth factor (TGF-β1), endothelin (ET-1) and hydroxyproline. Do biopsy specimens from some of the lung tissue, HE and MASSON staining, optical microscope. And line electron microscope specimens from some of the lung tissue. Results: The experimental animals exposed groups of lung tissue and serum of TGF-β1, ET-1, hydroxyproline compared with blank control group increased, the difference was statistically significant. 21 the skywave Health Tanzania treatment group compared with the same number of days exposed group of TGF-β1, ET-1, hydroxyproline reducing the difference was statistically significant. Optical microscope observation, lung tissue injury is aggravated with the experimental increase in the number of days, gradually increase the degree of inflammatory cell infiltration, alveolar septal fibrosis gradually formed late in the observation. Experimental animals of the treatment group gradually reduce the inflammation of lung tissue, mitigate the degree of fibrosis. Transmission electron microscopy, alveolar type II epithelial cells by the extent of the damage with the treatment of the number of days to extend gradually ease. Conclusion: Bosentan can reduce the release of inflammatory factors alleviate interstitial lung injury, in the treatment of paraquat poisoning caused by acute lung injury and pulmonary interstitial diseases have a certain effect.
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