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The Study on Virulence Differentiation and Genetic Diversty of Xanthomonas Oryzae pv .Oryzae Strains

Author: LiuXiaoHui
Tutor: ChengGuoYing
School: Huazhong Agricultural University
Course: Plant Pathology
Keywords: Xanthomonas oryzae Virulence differentiation Differential varieties Genetic Diversity
CLC: S435.111.47
Type: Master's thesis
Year: 2005
Downloads: 177
Quote: 1
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Abstract


A total of 72 strains the of bacterial blight representative bacteria in 17 countries such as China, the Philippines and Japan, as well as their single-cell lines, at the booting stage of rice, 16 IR24 carrier near-isogenic lines Jianye vaccination. The results showed that the bacteria in China and the Philippines, an average of 16 species of pathogenic bacteria rate is very close for about 42% of Japan bacteria pathogens was 31.2%, showing that the Chinese and Philippine strains overall pathogens rate than the Japanese strains of about 10% higher. IRBB5 (Xa5), Asominpri (Xa17), IRBB21 (Xa21) strains of the three countries with a high degree of resistance, IRBB7 (Xa7) high anti-China and Japan bacteria (antibacterial% gt; 97%), the Philippines bacteria 30 able to overcome its resistance; IRBB13 (Xa13), IRBB4 (Xa4) resistant to strains, but 45% of the strains in Japan, the Philippines, 35% of the strains overcome the resistance of these two varieties, IRBB3 (Xa3) resistance but most of the Philippines and Japan strains of China strains susceptible; These results 3 resistance resources of the country's exchange some reference value. 72 strains lesion / leaf length ratio of SAS 'new multiple range measured in 18 varieties (King Kong 30, Tetep, and 16 IR24 carrier near-isogenic lines), 18 varieties were divided into high resistance, disease resistance, resistance to, in a sense, susceptible, highly susceptible six groups, pick two sets of differential varieties: the first set of six varieties are: Feng of Kam (Xa18) IRBB14 (Xa14) , IRBB3 (Xa3), IRBB4 (Xa4), Java14 (Xa1, 3,12), IRBB5 (Xa5); identify varieties suitable for germs Daejeon natural groups virulence group is divided on the set differential hosts, 72 isolates were classified as 0,1,2,3,4,6,7,8,9,10 10 pathogenic group, 18 reactive; 0-6 virulence group was from weak to strong, the number of change; between 7-10 group, as well as in front of several groups, there is interaction cross-quality changes, such strains accounted for 34.4% of the tested strains. The second set of 10 varieties, respectively: Fung Kam (Xa18) XM5 (Xa19) IRBB14 (of Xa14) IRBB3 (Xa3), XM6 (Xa20) IRBB4 (Xa4), M41 (Xa15) Java14 (Xa1 , 3,12), IRBB5 (Xa5) Asominori (Xa17), the differential varieties more careful to distinguish between the virulence differences between bacteria, suitable for the identification and monitoring of physiological races. In this set of differential hosts, 72 strains were divided into 29 kinds of reactions 0,1,2,3,4,5,6,7,10,12,13,14,15,16,17,18 16 group type: 0 ~ 10 Species performance weak to strong changes in the quantity on the ground; between the group of 11 to 18 between the different strains within the group, and between groups with the preceding, there is interaction cross change in mass of the reaction, this strain accounted 45.9%. Determination of the pathogenicity of 64 strains isolated from Hunan, Guangdong, Yunnan and Hubei bacterial blight samples in 16 near-isogenic lines, varieties average 64 strains antibacterial% and the average lesion / leaf length ratio as a parameter, and 16 near-isogenic divided high resistance, disease resistance, resistance to, in a sense, a sense of disease and a high sense of six groups, IRBB7 (Xa7) and M41 (Xa15), antibacterial spectrum gt; 85% of the average lesion% lt; 16% for the high resistance group; IRBB5 (Xa5) Asominori (Xa17) IRBB21 (Xa21), 65% lt; antibacterial spectrum lt; 85% average lesion% lt; 25 % for the resistant group; IRBB2 the (Xa2) IRBB4 (Xa4) IRBB13 (Xa13), 45% lt; antibacterial spectrum lt; 65%, 35% gt; the average lesion% gt; 25%, as in the anti-group ; XM5 (Xa19), IRBB1 (Xa1), IRBB14 (Xa14),

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CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Crop pests and diseases and their prevention > Cereal crop pests and diseases > Rice pests and diseases > Disease > Transgression ( pass ) an infectious agent harmful > Bacterial blight
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