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The Effect of LPS on Proliferation and Apoptosis in HeLa Cell

Author: SunDongYan
Tutor: ShiYuXia；LinHong
School: Wuhan University
Course: Obstetrics and Gynaecology
Keywords: Cervical cancer cells Seaweed sulfated polysaccharide / apoptosis Heparanase bcl-2 NF-kBp65
CLC: R737.33
Type: Master's thesis
Year: 2005
Downloads: 102
Quote: 0
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Abstract


Objective: To explore the seaweed sulfated polysaccharides in vitro human cervical carcinoma cell line HeLa growth , apoptosis and cell cycle . Methods : HeLa cell, with different concentrations of LPS treatment with methyl thiazolyl tetrazolium blue colorimetry (MTT) Determination of cell proliferation , cell staining morphology of apoptosis , cell cycle changes recorded by flow cytometry bcl-2 and of NF-κBp65 gene expression , reverse transcriptase polymerase chain reaction to detect the expression of heparanase . Results : LPS so that the decline in cell proliferation , morphological changes of cell apoptosis and cell cycle by block , bcl- 2 protein , NF-κBp65 heparanase mRNA expression with increasing LPS concentration and duration of action the extension of reduced. Conclusion: seaweed sulfated polysaccharides significantly inhibited the growth of human cervical carcinoma cell lines , and by affecting the expression of bcl -2 , of NF-κBp65 gene protein expression to promote apoptosis of cervical cancer cells , seaweed sulfated polysaccharides also inhibit heparanase activity .

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Uterine tumors
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