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Of programmed cell death ( Programmed Cell Death PCD ) for organisms to maintain normal growth and development has a very important role in biological research has become a new hot spot . Higher plants PCD research lags behind the animal PCD , but has also made great achievements . In recent years, the plant PCD morphology, biochemistry, gene regulation, signal transduction research . Arabidopsis as a model plant has an irreplaceable important position in the field of botany . DAD1 (Defender against Apoptotic Death1) is a highly conserved apoptosis suppressor gene was first discovered in hamsters , in other vertebrates such as humans, mice and invertebrates , such as Xenopus laevis , nematodes have been found DAD1 same gene . Now in Arabidopsis, rice , corn , peas , barley , citrus, apples, tomatoes and other plants isolated the DAD1 homologous gene , but its function has not been depth . The DAD1 homologous genes AtDAD1 of Arabidopsis ( Arabidopsis thaliana Defender against apoptotic Death1 ) having a 345 nucleotide open reading frame (ORF, open reading frame), encoding a protein of 115 amino acids . Vertebrates at the amino acid level , it has a 49% (63% similar ) , with nematodes comparison , 48% (68% similar ) . We constructed a vector the Arabidopsis AtDAD1 overexpression , Agrobacterium-mediated transformation of Arabidopsis thaliana , comparing the difference between transgenic and wild-type plants phenotype differences , as well as their H the 2 O 2 < / sub > resistance different . The main results are as follows : to compare Arabidopsis AtDAD1 excess expression differences in phenotype of plants and wild-type plants , the development of transgenic plants slightly earlier than the wild-type plants , strong at the seedling stage in the wild-type . Specifically in the seedling stage, the transgenic plants leaves are slightly larger leaves than the wild-type plants slightly wider , the overall outline of the plant as compared to the wild - type large , flowering a little earlier . H 2 O 2 inhibition of seed germination experiments showed that when the medium does not contain H 2 O 2 when AtDAD1 transgenic plants and wild-type Arabidopsis germination time and germination rate nearly identical ; H 2 O 2 concentration , regardless of
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