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Objective: To study Acanthopanax saponin B (Sb) on rabbit ventricular sarcolemmal ATP-sensitive potassium channels ( sarcol K the ATR < / sub >) and ventricular muscle mitochondrial ATP-sensitive potassium channel (mito K ATR < / sub>) role. Methods : collagenase perfusion decomposition to obtain a single rabbit ventricular myocytes . Single rabbit ventricular myocytes divided into a control group , 10μmol / L Sb group 100μmol / L Sb group , using the whole-cell patch clamp technique different concentrations of Sb ??I K-ATP and its Ⅰ - ⅴ curve ; single rabbit ventricular myocytes were divided into a control group , 0.1 mmol / L Sb group , 0.5mmol / L Sb group , 1mmol / L Sb group , glibenclamide group , glibenclamide 1mmol / L Sb group , with rhodamine (Rhodamine 123) the dyeing , laser scanning confocal microscope ( multi-photon mode ) observed the impact of different concentrations of Sb ??mitochondrial fluorescence intensity . Results : Sb role sarcol K ATR : 10μmol / L Sb, 100μmol / L Sb , respectively, so that I K-ATP peak from 2.29 ± 0.26pA/pF rise to 6.87 ± 0.38pA/pF, 10.3 ± 0.55pA/pF, application of 3 μmol / L glibenclamide glibenclamide peak current decreased to 3.02 ± 0.23pA/pF , 3.74 ± 0.29pA/pF . 10μmol/LSb, 100μmol/LSb were Ⅰ - Ⅴ curve elevation , 100μmol/LSb groups raise more obvious application grid column Ⅰ - Ⅴ after the urea curves down . Sb no significant changes in mitochondrial fluorescence intensity of the mito K ATP role: the control group observed 15min . 0.1mmol / L Sb group 0.5mmol / L Sb group , 1mmol / L Sb group are visible mitochondrial fluorescence intensity increased after treatment , respectively ( 10.3 ± 6.78 ) % , ( 18.4 ± 5.51 ) % and ( 24.1 ± 7.64 )% , increase in fluorescence intensity based on the previous 5 minutes . 3μmol / L glibenclamide does not affect mitochondrial fluorescence intensity , but can block Sb mitochondrial fluorescence intensity . Conclusion : Sb on sarcol K ATP and mito K ATP , have open role , Sb is an effective monomer in Acanthopanax leaves saponins , open sarcol K ATP and mito K ATP , Acanthopanax produce an important mechanism of myocardial protection .
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