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Study on Molluscicidal Effect with Complex and LiuDaiShuiYangAn (LDS) and LDS Mechanism of Snail Control

Author: YuanYi
Tutor: DongHuiFen；XuXingJian
School: Wuhan University
Course: Pathogen Biology
Keywords: Schistosoma japonicum Oncomnalia hupensis histochemical enzyme complex LDS molluscicide effect mechanism of molluscicide
CLC: R184
Type: Master's thesis
Year: 2005
Downloads: 58
Quote: 1
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Abstract


This thesis was based on the advantage and disadvantage of the Niclosamide and Nicotinanilide in the snail control to explore combination commercials molluscicide of Nicotinanilide and niclosamide so as to increase molluscicisal effect. Nicotinanilide and niclosamide were complexed with the proportion of 1:1 (the short term is called complex as below). The result shows that the complex has a high effect and low dosage in the snail control. In order to improve the efficiency content of the complex, the chemical structure of Niclosamide and Nicotinanilide were unbind and recombine respectively, and then a crimson powder which has a good molluscicidal function was found in laboratory. However the spectrum of fluid mass show that the complex was occuped 1% only in the process of Niclosamide and Nicotinanilide were unbind and recombine. Further research indcated that more than 90% of crimson powder could be obtained only by actively Niclosamide. Thus a novel ramification of Niclosamide, which is called LDS was produced. Through initial study; the result showed that the molluscicidal effect and dosage of LDS was the same with the complex. As for the impact in the histochemical of the snail by LDS was also with no difference compared with Niclosamide. Currently the research project of development and application of LDS is arranged in national high technique research development plan (it is called 863 plan).This thesis was the result of the initial and pilot study of the research project. Implementing the research project and reaching the purpose of the project are not only obtaining property right in our country and also could play an important role of resolving the status of applying singleness and costliness molluscicide. 1. Molluscicidal effect of complex of nicotinanilide and niclosamide produced in different manufactoriesUnder the room temperature 23-25℃ in Lab, 0.2mg/L of the complex was used by immersion method for 48h in Lab. The mortality of the snails in the group ofHuai-Nan, Wu-Jiang and Si-Chuan was 93.3%, 94.7% and 95.4% respectively; 0.4g/m2 of the complex was used by spraying method for 48h in Lab. The mortality of the snails in the group of Huai-Nan, Wu-Jiang and Si-Chuan was 97%, 96% and 96% respectively. The ratio of snails left water in the complex group was decreased 71.9% than that of nicotinanilide group. The ratio of snail left water has a significant differences between complex group and nicotinanilide groups(P<0.01). As for acute toxicity offish, the complex group was reduced about 50% than that of niclosamide group.2. Molluscicidal effect with immersion method by LDS in LabUnder the room temperature 20°C,25°C and 30°C in Lab, 0.4mg/L of LDS was used by immersion method. The mortality of the snails for 48h in three temperature group was 96.7%, 100% and 100% respectively. The ratio of snails left water in the complex group was decreased 13.3% than that of nicotinanilide group.3. LDS mechanism of snail conrolUnder the room temperature 25 °C in Lab, O.lmg/L of LDS was used to contact the snails by immersion method for 24h in Lab. Then to observe the change of histology of the snails.the result showed that there is damnification between the experimental and positive control groups. After 24h the activation impact of SDH, LDH, NOS, CHE, Mg2+-ATPase in the snail was displayed respectively by histochemical techniques. Initial result indicated that there is no difference between LDS and Niclosamide on the activation impact in five enzyme of the snail. Therefore it speculated that the mechanism of molluscicide is the same with LDS and Niclosamide.

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