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Observe and explore its possible mechanism of action of oxytocin (oxytocin, OT) seizures in rats effect . Penicillin -induced rat model of epilepsy , were divided into A , B two parts . Part A as part of electrophysiological experiments , the animals were randomly divided into A1 , A2 , A3 , A4 , A5 , A6 , a total of 6 group ( n = 6 ) , A1 group for the control group , A2, A3, A4 group respectively intracerebroventricular injection of oxytocin 1ng, 10ng, 100ng, A5 group oxytocin receptor antagonist [ d ( CH 2 < / sub >) 5- OVT ] 1μg A6 group was injected with [ d ( the CH 2 ) 5-OVT] 1μg oxytocin 100ng. Observe the brain frontal cortex and hippocampal CA3 EEG epileptic wave firing frequency , amplitude , and the change in the form of the discharge . Part B for immunohistochemistry experiments part , divided into B1 , B2, B3 , of 3 group B1 group intraperitoneal injection of saline control group , the the B2 group for intraperitoneal injection of sodium penicillin epileptogenic group B3 oxytocin treatment group , intraperitoneal injection of oxytocin 20μg first 30min after intraperitoneal injection of penicillin induced epilepsy . Groups of brain tissue heat shock protein 70 (Heat Shock Protein 70, HSP70) expression changes detected by immunohistochemical methods . The results show that A4 animal intracerebroventricular injection of oxytocin 100ng, can significantly increase the the the epileptic wave frequency (39.67 ± 6.19 times / min , t = 5.777 , P LT ; 0.01 ) and amplitude ( 540.24 ± 71.12μV , t = 3.168 , p lt; 0.05 ) ; A3 group was injected with oxytocin 10ng, the epileptic wave of magnitude increase ( 538.90 ± 55.95μV , t = 4.004 , p lt; 0.05 ) ; the A6 group pretreated with [d ( CH 2 ) 5-OVT] epileptic discharge wave frequency and amplitude , no significant change. Group B1 brain tissue is almost no expression of HSP70 , B2 groups HSP70 expression was strongly positive than group B2 , B3 group HSP70 expression weakened . The experimental results show that intracerebroventricular injection of certain doses of oxytocin enhances experimental epilepsy in rats epileptiform discharges , oxytocin receptor antagonists can block the facilitatory effect , suggesting that oxytocin may be through its receptor mediated role to play to promote epilepsy . Oxytocin can also aggravate seizures induced neuronal damage , and its mechanism may be associated with reduced expression of HSP70 .
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