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The Study of Apoptosis and Insulin Signaling of Rat Pancreatic Islet Cells Caused by TNF-α

Author: WangGongLing
Tutor: WenFang
School: Nanhua University
Course: Internal Medicine
Keywords: TNF-α Islet cells Apoptosis Bcl-2 Bax The insulin receptor substrate-1 The insulin receptor substrate-2 The glucose transporters sub -2
CLC: R587.1
Type: Master's thesis
Year: 2006
Downloads: 115
Quote: 0
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Abstract


Objective To investigate the first part only TNF-α -induced SD rat islet cell apoptosis induced in vitro SD rat islet cell apoptosis and Bcl-2 and BaxmRNA expression levels of tumor necrosis factor -α (TNF-α) . Methods Male SD rat pancreas by collagenase P digestion Ficoll 400 , was purified islets, at 37 ° C in 5 % CO 2 , RPMI 1640 culture medium and cultured for 7 days , paved monolayer with different concentrations of TNF - α ( respectively for 0,10,30,50 ng / mL) were incubated for 24 hours. Applied deoxy nucleotide terminal transferase -mediated nick end labeling (TUNEL) method to detect islet cell apoptosis rate ; application of semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) to detect islet cells , Bcl-2 and BaxmRNA expression levels changes. The number of islet cell apoptosis 10ng/mLTNF-α group with the control group showed no significant difference ( P > 0.05 ) groups 30ng/mL and 50ng/mLTNF-α islet cell apoptosis number of the control group increased significantly (P <0.05 ). Bcl-2 mRNA expression levels can increase 10ng/mLTNF-α to 30ng/mL and 50ng/mLTNF-α can significantly reduce the level of expression of Bcl-2mRNA . 10ng/mL, 30ng/mL , and 50ng/mLTNF-α are to make BaxmRNA expression level increased . Conclusion (1) TNF-α was concentration -dependent induction of islet cell apoptosis in vitro . (2) 30ng/mL , and 50ng/mLTNF-α can reduce the the islet cells Bcl-2mRNA expression levels . 10ng/mL, 30ng/mL , and 50ng/mLTNF-α are to make BaxmRNA expression level increased . TNF-α -induced apoptosis of islet cells may reduce and BaxmRNA expression and islet cell Bcl-2 mRNA expression levels

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CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetes
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