Dissertation > Excellent graduate degree dissertation topics show

The Inhibition of Human Vascular Endothelial Growth Factor Expression by the Technology of RNA Interference in Non-small Lung Cancer

Author: GaoYunBo
Tutor: ZhangPing；Qian
School: East China Normal University
Course: Biochemistry and Molecular Biology
Keywords: Vascular endothelial growth factor angiogenesis siRNA expression cassettes gene therapy
CLC: R734.2
Type: Master's thesis
Year: 2006
Downloads: 55
Quote: 0
Read: Download Dissertation

Abstract


Lung cancer is a severe disease that has a great danger impact on human health. Despite great improvement of medicial treatment, there is no more than 10% livability when getting a lung cancer. The biological character of lung cancer is the metastasis, which is also the first reason of the treat failure and high mortality. The angiogenesis is the basis of the tumor growth and metastasis, how to inhibit the angiogenesis is the important method in the treatment of cancer. As a cytokine, vascular endothelial growth factor (VEGF), that is an important role on the vascular growth. Many research revealed that VEGF is a key factor to affect the vascular growth and the metastasis for the lung cancer. So the inhibition of the VEGF expression will be helpful to arrest the growth and metastasis of the lung cancer. So we select the gene of VEGF as the interest gene and try to find an effective method for tumor therapy by PCR-based siRNA expression cassettes to inhibit VEGF expression in non-small cell lung cell A549.Gene carrier is an important part in the gene therapy. There is some defect for viral and non-viral carrier used in recent treatment of gene therapy. In our experiment, we select a non-viral carrier from a Japanese company NDT for our future experiment. We had got good transfection efficiency up to 40% by improving the transfection condition. Then we designed the siRNA sequence targeting the CDS region of VEGF, then utilized PCR based strategy for rapid synthesis of siRNA expression cassettes. The PCR products are directly transfected into A549 cells or injected into nude mice model resulting in functional expression of siRNAs. We used EGFP as

Related Dissertations

  1. Polyethylenimine Conjugated Stearic Acid-g-chitosan Oligosaccharide Micelles for Antitumor Gene Therapy,R450
  2. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Immature Mouse,S865.13
  3. The Construction and Evaluation of a Novel Non-viral Gene Transfection System and Its Application in Mesenchymal Stem Cells Gene Recombination,R346
  4. The Expression of MMP-2、 VEGF and Ki-67 and Human Glioma Grade,R739.41
  5. Effect of β-elemene Combined with Radiotherapy on the Angiogenesis of Lung Adenocarcinoma Xenograft Tumor in Nude Mice,R734.2
  6. The Research on Treatment of Lentivirus-mediated hVEGF-165 Gene Transferring Mesenchymal Stem Cells on Rat Hind Limb Ischemia,R329
  7. Inhibition of Conventional Chemotherapy Combined with Metronomic Chemotherapy on Breast Cancer Xenografts in Nude Mice,R737.9
  8. The Eukaryotic Expression Vector Construction of pIRES2-EGFP-hIL-12-HSP70 and Its Influence on the Growth of 7402 Liver Cancer Cell,R346
  9. BMP2 and VEGF165 Genes Transfected Rat Bone Marrow Mesechymal Stem Cells Simultaneity Expression,R329
  10. Expression of TNF-α, MMP-9 and VEGF in BALF of Rats with Emphysema and Their Mutual Relationship,R563.3
  11. Improvement of the Result of Autologous Fat Transplantation in Facial Recontouring Surgery by Using "Cocktail" Therapy,R622
  12. The Experimental Study of Antiangiogenic and Induction of Apoptosis by Maximum Tolerated Dose Combined with Low-dose Cyclophosphamide in Breast Cancer,R737.9
  13. Maximum Tolerated Dose Combined with Metronomic Chemoterpy on Proliferation and Angiogenesis in Treatment of Breast Cancer,R737.9
  14. Influence and Significance of MMP-2 and VEGF of Hemangioma Endothelial Cells in Vitro Culture by Propranolol,R739.5
  15. Fundamental Research of the Function of Angiogenesis Inhibitors to Colon Cancer Stem Cells in Mice and Analysis of Related Mechanism,R735.35
  16. The Investigation of Relationship between Therapeutic Effect and Prognosis and Changes of Peripheral Blood Vascular Endothelial Growth Factor and Metallothionein in Patients with Advanced Lung Cancer,R734.2
  17. Clinical Analysis of Multifocal Papillary Thyroid Carcinoma,R736.1
  18. Study on the Correlation btween Serum, Aqueous Humor VEGF, IL-6 Levels and the Neovascular Glaucoma,R775
  19. The Analysis of the Growth Factor Related with the Secondary Glaucoma in the Anterior Champer of Silicone Oil-filled Eyes,R779.6
  20. Vascular endothelial growth factor on endothelial cells concentration of magnesium ions,R965
  21. A Preliminary Exploration of Laboratory Diagnosis and IVF Improvement Protocol on Endometriosis,R711.71

CLC: > Medicine, health > Oncology > Respiratory system tumors > Lung tumors
© 2012 www.DissertationTopic.Net  Mobile