|
This paper the use of high-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) and principal component analysis (PCA) is a combination of rat liver fibrosis model of human pancreatic cancer tumor-bearing nude mice model rats with chronic pancreatic metabolites studied characteristics of the inflammatory model . Found in rat liver fibrosis model , fibrotic lesions makes the increase in liver fat content : glucose , glycogen , taurine and inositol content relative decrease ; relatively phosphorylcholine (PC) , choline (Cho), glycerophosphocholine (GPC) reduced. In addition , with the deepening of fibrosis , fat content , there is a first significant rise, then a slow downward trend ; while other metabolites did not change significantly between the different degrees of fibrosis . In human pancreatic cancer tumor-bearing nude mouse model study , phosphorylcholine (PC) as the internal standard reference , combine PCA results found cancerous relative to normal samples , leucine / isoleucine , valine , lactic , alanine, glutamic class , choline , taurine , and some sugars relative increase , while glycerophosphocholine (GPC) , and δ3.66 at unknown peaks relative decrease . With the continuous increase of radiation dose in the comparative study of the cancerous tissue before and after different doses of radiation therapy , the tumor volume decreasing from the spectra of simple comparison found no significant metabolic changes , but the PCA results seem to show the different stages of certain trend by taurine and choline - containing compound content caused by a change . In rats with chronic pancreatitis model , PC as an internal standard , relative to normal tissue visible chronic pancreatitis , GPC elevated , TMAO Bet Glx, alanine, glycine , and δ3.88 at an unknown compound is relatively reduced. Body is not the same due to pancreatitis and cancerous tissue exists between the species differences in the normal rat and nude mice , such as the compounds of the choline , taurine , glycine , acetic acid , δ3.88 at an unknown compound and the like , on pancreatitis and carcinogenesis caused some difficulties , so the remaining work will need to be a follow-up study .
|