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Preparation of MMS Carriers and Their Application in the Immobilization of Enzymes

Author: LuoKaiKun
Tutor: GongYueFa；PengHong
School: Huazhong University of Science and Technology
Course: Organic Chemistry
Keywords: Lipase Laccase Magnetic microspheres Composite carrier Immobilized Biocatalysis
CLC: O629.8
Type: Master's thesis
Year: 2005
Downloads: 459
Quote: 6
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Abstract


Since the sixties of last century, the immobilized enzyme technology has developed very quickly Immobilized Enzymes research has become an important part in the immobilization technology. The magnetic microspheres carrier because of its unique structure and function has attracted the attention of people, it is generally composed of two parts of the magnetic core and outside the core of the polymer shell wrapped. The introduction of organic functional groups by simple chemical modification method and the silicone embedding outer magnetic core, thereby preparing a new kind of magnetic microspheres with the carrier, and evaluation of such carriers for immobilized laccase and lipase performance. First, the use of the mercapto compound is easy and Fe (OH) 2 , Fe (OH) 3 reagent characteristics the magnetic Fe 3 were prepared by a coprecipitation method O 4 material (M1 carrier), surface containing carboxylate magnetic Fe 3 O 4 material (M , the the magnetic Fe 2 -type carrier) and the surface containing amino 3 O 4 material (M3 carrier). Recycling characteristics of the silane coupling agent can be firmly bonded with an inorganic compound, were prepared by embedding M5 M4 and surface of the surface containing an amino group containing an ester group-type magnetic silica composite carrier. Secondly, the use of M1-M2 and M3 magnetic carrier laccase immobilized. The results showed that: the coupling efficiency of the three carriers are respectively 9.62%, 62.1% and 64.1%; load were: 0.0415 mg / g 0.457 mg / g and 0.480 mg / g. Thus, the M3-immobilized laccase better. Further studies showed that: M3 vector at pH6.8, the ion concentration of 25 mmol.L -1 , immobilized laccase activity. But, in general, M1, M2-M3-immobilized laccase activity is not high, the application of little value. Used in the experiment laccase by higher production laccase activity bacteria culture obtained. In order to get a higher activity laccase, the paper also studied the impact of various culture conditions on fungal laccase production capacity, and the resulting paint enzymatic and catalytic properties. The experimental results show that: the optional filter out bacteria from the laccase production fungi (L1), phenol as an inducer under 30 ° C PYGM medium cultured for 7 days, the highest production activity. The laccase optimum pH 8.0, optimum temperature is 33 ° C, Cl-its inhibition. The laccase concentration of 50 to 250 mg / L of phenol a good catalytic degradation effect, and maximum degradation rate of more than 60% within 24 h. Again, M4 and M5 magnetic carrier lipase immobilized research. The experiments show that: M4-immobilized lipase coupling efficiency above 86%, the activity recovery of between 35% -50%. M5 immobilized lipase coupling efficiency of 88.1% activity recovery was 59.4%. Further study showed that

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CLC: > Mathematical sciences and chemical > Chemistry > Organic Chemistry > Natural compounds > Enzymes, hormones,
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