Dissertation > Excellent graduate degree dissertation topics show

Preparation, Characterization and Application of Novel Fluorescent Chitosan Nanoparticle Probe

Author: ZhaoJiaZuo
Tutor: ZuoJianMin
School: Zhejiang University
Course: Analytical Chemistry
Keywords: Chitosan microspheres Ionic crosslinking Fluorescent probe FITC Surface Modification IgG
CLC: O636.1
Type: Master's thesis
Year: 2006
Downloads: 387
Quote: 0
Read: Download Dissertation

Abstract


In this thesis, chitosan as raw material, the use of hydrogen peroxide degradation Preparation of low molecular weight chitosan; ionic crosslinking Preparation of Chitosan nanoparticles labeled with fluorescein isothiocyanate (FITC) The micro- ball, Chitosan Nanoparticles as Fluorescence Probes; paper further dispersion of chitosan nanoparticles to explore nanospheres stable, good dispersion conditions, and use a variety of surface modifiers on chitosan sugar nanospheres subjected to surface modification in order to improve its stability; coupling method of the the chitosan nano fluorescent probe with the biologically active substance, and applied to a fluorescent immunoassay detection of human immunoglobulin antigen. This thesis consists of five chapters. The first chapter introduces the fluorescent probe, the structure and properties of chitosan, chitosan nanoparticles preparation method and microsphere surface modification method. Chapter II Preparation of low molecular weight chitosan with hydrogen peroxide degradation, the effects of the degradation of the influencing factors. Ionic crosslinking method, sodium tripolyphosphate (TPP) as the crosslinking agent, chitosan nanoparticles. Explore the influencing factors into a ball, and the the nanospheres performance characterization. The experimental results showed that the molecular weight (Mr) of about 60,000 low molecular weight chitosan, the concentration of the acetic acid solution of chitosan is 0.5% (w / v) 0.25% (w / v), the concentration of the crosslinking agent TPP The chitosan solution and TPP solution is preferably a volume ratio of 3:1 was prepared microspheres performance. The prepared chitosan microsphere particle size of about 40nm, spherical, more uniform particle size, the better dispersibility. Chapter homemade nano-chitosan microspheres as a substrate, the use of chitosan chain amino and fluorescein isothiocyanate-reactive Preparation of Chitosan Nanoparticles as Fluorescence Probes. Explores the impact of nano fluorescent probe preparation conditions. Experimental results show that the FITC amino in alkaline medium with a solid, obtained Chitosan Nanoparticles as Fluorescence Probes relatively stable and difficult to photobleaching. The fourth chapter discusses the the chitosan microspheres stable conditions. The results showed that the chitosan microspheres in pH = 2-6 remained stable in the pH = 4 stability when the best monodisperse best. Appropriate ionic strength can improve the stability of the nanospheres. Discussion with a silylating reagent of chitosan microspheres surface modification, in order to improve the stability of the method. The fifth chapter discusses the chitosan nano fluorescent probe with the biologically active substance coupling method, coupling efficiency comparison of several methods, found glutaraldehyde as a coupling reagent, the coupling effect is best. Chitosan Nanoparticles as Fluorescence Probes labeled IgG antibodies to detect trace amounts of IgG antigen good detection results.

Related Dissertations

  1. Preparation of Crop Stalk-Based Sorption Materials with High Performances and Their Applications for Pah Removal from Water,X712
  2. Synthesis and Biocompatibility of different types of hydrophilic surface - modified polyurethane,R318.08
  3. The Effects of RIP on the Immune Function of Renewal Rats Without Colostrums,S853.7
  4. Synthesis and Analysis of Coumarin-fluorescent Probes,O657.3
  5. Further Evaluation of Recombinatn SjLAP and SjFBPA for Detecting Antibodies Against Schistosoma Japonicum,R532.21
  6. Polyaniline / short fiber reinforced natural rubber properties,TQ330.1
  7. Phosphate modification of nano- TiO 2 Photoelectrochemical Properties of the,TB383.1
  8. Construction of Multi-responsive Gene Vector Via Layer-by-Layer Assembly,TB383.1
  9. Study on Laser Surface Modification of Two Kinds of Super-alloys,TG174.4
  10. Atmospheric Pressure Plasma Surface Modification of Carbom Fibers and Numerical Simulation of the Discharge,TQ342.742
  11. Study on Modification of PBO Fibers by Atmospheric Pressure Plasma,TS195.6
  12. The Regulation and Research of Mechanism of "Plus Sijunzi Decoction" in Immune System of the Aging Rat,R285.5
  13. Surface Modification of Poly (ε-caprolactone)s and Its Influence on Cell Behaviour,R318.08
  14. Stainless steel fuel cell bipolar plate and its experimental and simulation,TM911.4
  15. Nuclear Power Plant Steel Milling Surface Integrity Study,TG54
  16. Atmospheric pressure pulsed RF plasma discharge for high strength and high modulus polyethylene fiber surface modification,TQ342.61
  17. bFGF chitosan microspheres and promote Schwann cell proliferation studies,R651.2
  18. Study on Implant Material of Carbon/Carbon+Hydroxyapatite Coating (C/C+HA) Composites,R318.08
  19. Research on Powder Sintering Preparation and Properties of Porous Titanium,R783.1
  20. Effect of Self-control Exercise on Immunoglobulin and Serum Complement in Malignant Tumor Patients,R730.5
  21. Investgation and Analysis on Epidemiological Features and Immunization Status of Epidemic Mumps in Shihezi City,R181.3

CLC: > Mathematical sciences and chemical > Chemistry > Polymer chemistry ( polymer ) > Natural polymers ( polymer ) > Polymer polysaccharides
© 2012 www.DissertationTopic.Net  Mobile