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Study on Degradation of Chlorophenol by Dominant Mixed Bacteria

Author: GengLiang
Tutor: LuGuangHua
School: Hohai University
Course: Environmental Science
Keywords: Chlorophenol Complex bacteria Tolerated Biodegradable Cometabolism
CLC: X703
Type: Master's thesis
Year: 2006
Downloads: 186
Quote: 1
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Abstract


The return sludge from Yangzi Petrochemical industrial sewage treatment plant wells to extract the active sludge samples, chlorophenols as the sole carbon and energy tolerance composite bacteria were isolated, cultured and domesticated. Consists Flavobacterium (Flavobacterium), Acinetobacter species (Acinetobacter), Alcaligenes (of Alcaligenes), sticks (Mycopiana) Acinetobacter and Pseudomonas species (Pseudomonas) were identified. Bacterial growth inhibition experiments show that bacterial of composite bacteria isolated mixed chlorophenols tolerated than natural water bodies (Yangtze), and after 2 - chlorophenol selective medium domestication, composite bacteria to poison tolerated significantly improved. Domesticated complex bacteria-degrading microorganisms, use of laboratory simulation method to study the characteristics of three single-Chlorophenol biodegradation. Results demonstrate that the three single chlorophenols fall isomers, but chlorine atom substituted in different positions, their biodegradability have a greater impact, degradation balance after removal rate of the order of: 3 - chlorophenol gt; 2 - chlorophenol gt; 4 - chlorophenol. 3 - chlorophenol target contaminants, research compound exposure concentration composite inoculation, water pH value and other conditions the rate of biodegradation of the target compound and the final removal rate. The results show that the complex bacteria strong adaptability, can be adapted to the pH from 3.5 to 9.5, a wide range of environmental pH changes. Reduce the concentration of pollutants in the water, and composite bacteria inoculum size, and can improve the efficiency of biodegradation of the target compounds. Add the growth substrate chlorophenols biodegradable characteristics have an important impact. The phenol join conducive to microbial utilization of 3 - chlorophenol. Microbial degradation of phenol as a growth substrate for priority, followed by 3 - chlorophenol was only decomposition, and increased metabolic efficiency, 3 - the chlorophenol degradation efficiency increase of concentration of phenol added to improve. Compared with the situation alone, 2 - chlorophenol and 3 - under the conditions of coexistence chlorophenol, poison lag phase and reached the degradation of the balance of the time has a significant hysteresis the two chlorophenols half-life was significantly longer, final removal rate also have been reduced. Visible, of two chlorophenol coexist on the composite bacteria degradation inhibition, their combined effects of an additive effect. Biological degradation of chlorinated phenols dynamics study found that 3 - chlorophenol degradation process has produced two major intermediate metabolites exist obviously have accumulated degradation process. Its metabolic pathway is presumed to: ortho oxidation occurs under the action of a single oxidase generates chloro catechol; chloro catechol open loop and then in the ortho position under the action of 1,2 - dioxygenase; within the esterification process, the release of chloride ion, and is oxidized to maleic acid acetic acid, and finally into the Krebs cycle.

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CLC: > Environmental science, safety science > Processing and comprehensive utilization of waste > General issues > Wastewater treatment and utilization
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