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[Objective ] environmental anti-androgen (environmental antiandrogen, EA) , belongs to environmental endocrine disruptors , which were originally present in the environment , through a variety of molecular mechanisms produce antagonistic androgen response role thus inhibiting male differentiation and developmental processes chemicals, and most of the androgen receptor (AR) antagonists. EA include some pesticides and their metabolites , non-steroidal drugs , plasticizers , pesticides and their metabolites largest proportion , but also in the areas of research focus . The pyrethroid pesticide widely used mainly as an insecticide in the country due to its broad-spectrum, high efficiency, low residue , human toxicity , etc. , the exposed population is large , such pesticides in recent years, becoming the anti-androgen pesticides sieve the focus of the inspection , in order to protect the reproductive health , it is necessary to study antiandrogenic pyrethroid possible . Belonging to such permethrin was previously reported in vivo Hershberger test in anti-androgen activity , this study selected permethrin, as well as three-dimensional chemical structure similar cyfluthrin , cyfluthrin , cypermethrin , beta-cypermethrin , bifenthrin 6 pesticides by in vivo the Hershberger test with in vitro stable transfected reporter gene test is a combination of methods to explore the six kinds of pyrethroid anti-androgen effect and its possible mechanism for a comprehensive evaluation of the safety of such pesticides sexual provide some scientific basis . [Method ] in vitro androgen dependent gene transcription activation test using stable transfection of luciferase reporter gene in human breast cancer cell lines of MDA-kb2 cells , the cell lines to the U.S. Environmental Protection Agency 's Wilson
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