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Research of Genes Involved in Myeloid Neoplasms with Aberration of Chromosome 3

Author: ZhangMeiRong
Tutor: XiaoZhiJian
School: Peking Union Medical College , China
Course:
Keywords: myeloid leukemia chromosome translocation chromosome 3 gene
CLC: R733.7
Type: Master's thesis
Year: 2006
Downloads: 45
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Abstract


ObjectiveTo explore the clinical features of myeloid neoplasms patients with rare aberrations of chromosome 3 and the possible molecular mechanisms of these myeloid neoplasms.Method1. 4 patients with MDS whose cytogenetic analysis showed t(1;3)(p36;q21) were reported. The expression level of two transcription forms (PR-containing form MEL1 and PR-lacking form MEL1s) of MEL1 gene in normal fetus tissues, 2 healthy donor bone marrows, 10 leukemia cell lines, and bone marrows from 3 MDS patients with t(1;3)(p36;q21) were detected by reverse transcription polymerase chain reaction (RT-PCR).2. One case AML secondary to MDS with t(3;5)(q25;q34) was reported. The expression of NPM-MLF1 fusion gene and MLF1 gene were detected by RT-PCR. NPM gene mutation was analyzed by PCR combined with sequencing. The EVI1 and MDS1/EVI1 genes expression were examined by fluorescent Real-time PCR. The subcellular localization of wild MLF1 and NPM-MLF1 proteins were determined by immunohistochemistry staining.3. One case CML patient with t(3;21)(q26;q22) in blastic crisis was reported. Genes involved in t(3;21)(q26;q22) chromosome translocation were analysed by RT-PCR combined with sequencing.4. The expression level of EVI1 and MDS1/EVI1 genes in patients with myeloid tumors were detected by SYBR Green I real-time RT-PCR.Results1. MEL1 gene was mainly expressed form in the detected normal fetus tissues, healthy donor bone marrows and leukemia cell lines. t(1;3)(p36;q21) MDS bone marrows mainly or only expressed

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CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia
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