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The Effects of PNS on Fibroblasts Cultured from Human Hypertrophic Scar in Vitro

Author: YaoHeng
Tutor: LiShiRong
School: Third Military Medical University
Course: Surgery
Keywords: PNS hypertrophic-scar fibroblasts myofibroblasts collagen cell circle TGF-β1 α-SMA
CLC: R285.5
Type: Master's thesis
Year: 2005
Downloads: 101
Quote: 0
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Abstract


Hypertrophic scars represent an aberration in the fundamental processes of wound healing. It is one of the most common pathological phenomenon seen after wound healing of trauma, especially in deep burned patients. Excessive proliferating fibroblasts and overabundant wound matrix responsible for raised, red and inflexible scar tissue, which causes itching and pain,and might lead to serious functional and cosmetic problems for the patient. The mechanism of its formation is still in debating, by now we reach consensus on it, the fibroblasts play an important role in the process. Over the years, we keep on searching for a reliable surgical excision, intralesional corticosteroids, injectionsm, radiation ,laser surgery, cryotherapy and other medication, combined ways can get better results than the sole one. But unfortunately have not yet been successful in preventing and treating hypertrophic scar. So to seek the effective way and medicine for prevention and cure HS is a very important subjection on plastic surgery science. To provide experimental bases for prevention and cure HS by study PNS’s inhibitory action to HSFb.Methods:1. HE staining was applied to observed the morphological changes of HSFb2. To observe the changer of cell circle the human hypertrophic scar fibroblasts were cultured in vitro. By the means of flowing cytometry ;3. The effect of PNS on HSFb proliferantion was examined by MTT colorimestric cell proliferation- assay.4. Having established fibroblast populated collagen lattice (FPCL) by culturing the human hypertrophic scar fibroblasts in vitro. And then to add 400ug/ml PNS, finally to observe the influence of PNS on the contraction indexes.5. Evaluating the expression of TGF-β1,α-SMA in the human hypertrophic scar fibroblasts were cultured invitro after 12, 24, 48 and 72 hours with immunohistochemistry SABC method.

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