Dissertation > Excellent graduate degree dissertation topics show

Mechanism and Effects of Human Ribonuclease Inhibitor on Insulin-liked Growth Factor-Ⅱ in Cancer Cell

Author: FanJianHui
Tutor: CuiXiuYun
School: Dalian Medical University
Course: Biochemistry and Molecular Biology
Keywords: ribonuclease inhibitor interaction of protein insulin-liked growth factor tumor Co-Immunoprecipitation
CLC: R341
Type: Master's thesis
Year: 2006
Downloads: 41
Quote: 0
Read: Download Dissertation

Abstract


Ribonuclease inhibitor (RI) is an acidic cytoplasmic protein with a 50 kDa estimated molecular weight. RI can inhibit the activity of ribonuclease A (RNase A) by tight combination with it (Ki=4.0×10-14M), and RI can also inhibit Angiogenin (Ang) effectively. Angiogenin has a robust capacity to induce blood vessel formation in vivo and in vitro, growth of tumor correlated with new vessels, so it in favour of the existence of Ang. RI suppress the growth of tumor cells by inhibiting the activity of Ang. RI is constructed almost entirely of leucine-rich repeats. Such repeats have been identified in more than 100 proteins that exhibit a wide range functions, including cell-cycle regulation, DNA repair, extracellular matrix interaction, and enzyme inhibition. RI located on the chromosome 11p15.5. Insulin-like growth factor -2 (IGF-2), on the same position, is mitogen. Not only IFG-2 can regulate cell growth, differentiation, apoptosis, and transform by paracrine and autocrine, but it is an important stimulating factor in signal conduction pathway. Unbalance of any process of above makes cell proliferation out of control, therefore, induces canceration. There are evidences that IGF-2 increases cell proliferation, inhibits apoptosis and raises local infiltration and transfer in many tumor cells. RI spare the nearby same position of human chromosome with IGF-2 , however, they have contradistinctive contribution. For above all, we presume that they may have some unknown interaction. We expect to further study the important role of RI in signal conduction pathway, and then provide important theory for RI’s contribution of anti-tumor and anti-vascularization.The purpose of this study is to discuss the effect to IGF-2 by over-expressed RI in eukaryotic cell line, and to find out the relationship between RI and IGF-2, so as to illustrate the mechanism of anti-tumor and anti-oxidation of RI. This experiment established a infection of eukaryotic cell line: Human breast carcinoma cell line MCF-7, by retrovirus carrying

Related Dissertations

  1. The Regulate and Controlof Slug on P53-MDM2 System,R730.2
  2. Design, Synthesis and Antitumor Activity of thalidomide derivatives,R965
  3. Atovaquone analogues Design, Synthesis and antitumor activity of,R914
  4. Study on the Preparation of the Low Molecular Weight Fuciodan from Sargassum Henslowianum (C.Agardh) and Anti-tumor Activity,TS254.9
  5. Design, Synthesis and Antitumor Activities Study of Quinoxaline-1,4-dioxide Derivatives,R914
  6. The Study on Anti Tumor and Anti-oxidation Activities in Vitro of Extracts from Oxalis Corniculata L,R285
  7. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  8. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  9. The Clinic Value of Diffusion Weighted Whole Body Imaging with Background Body Signal Suppression (DWIBS) Compared with hPET/CT,R445.2
  10. Therapeutical Effects of Diammonium Glycyrrhizinate Lipid Ligand on Nonalcoholic Steatohepatitis and Its Partly Mechanisms of Action,R575.5
  11. Observastion on Histomorphology, Ultrastructure and Expression of TNF-α, IL-1β of Unbalance of Dynamic and Static Forces Cervical Degeneration Disc in Rat Model Abstract,R681.55
  12. The Clinical Value of Detection of Serum TPS, CEA, CYFRA21-1, NSE for Diagnosis in Patients with Lung Cancer,R734.2
  13. Further Studies on the Side Effects of Imatinib in the Treatment of Patients with GIST,R735
  14. Effects of the Therapeutic Communication System Intervention on the Postoperative Gynecological Malignant Tumor Patients with Depression,R473.73
  15. Diagnostic Value of Multiple Tumor Marker Protein Biochip for Patients with Esophageal Carcinoma and Cholangiocarcinoma,R735.1
  16. The Experimental Study of Semiconductor Laser Radiation and 5-FU Sustained Release Repressed Oral Malignant Tumor Cell,R739.8
  17. Recurrent Ovarian Cancer Surgery Again with Surial Analysis of Relevant Factors,R737.31
  18. Urinary Inflammatory Myofibroblastic Tumor Clinicopathological Analysis and Literature Review,R737
  19. Combined Detection of Tumor Markers in the Diagnosis and Treatment of Lung Cancer to Determine the Clinical Significance,R734.2
  20. Study on the Interaction between Functionalized Nanodiamond Materials and Cells,R96
  21. Anti-gout Capsules on the Treatment of Gouty Arthritis and Its Mechanism,R259

CLC: > Medicine, health > Basic Medical > Human biochemistry, molecular biology
© 2012 www.DissertationTopic.Net  Mobile