Dissertation > Excellent graduate degree dissertation topics show

The Effect of Oocyte Maturation Inhibitor on Porcine Oocyte Maturation

Author: ZhouHongBin
Tutor: LiYueMin
School: Southwestern University
Course: Clinical Veterinary Medicine
Keywords: porcine oocyte inhibitor meiosis acivation
CLC: S828
Type: Master's thesis
Year: 2006
Downloads: 113
Quote: 2
Read: Download Dissertation

Abstract


In Comparison with cattle, goat and other animals oocytes in vitro maturation , porcine oocytes in vitro maturation is poor in quality .which reflects in these aspects : poor cytoplasmic maturation highly polyspermy and poor embryo development. In recently years , many scholars studyed adding inhibitor to maturation medium to improve cytoplasmic maturation. Even though inhibitors contribute little to nuclear maturation, they can inhibit the nuclear maturtion and make oocytes stay at GV stage, which may promote nuclear and cytoplasmic maturation synchronization. Moreover, inhibitor cause oocytes nuclear maturation to concentrate and decrease aging oocyte proportion. In this experiment, we mainly detected and recorded oocytes germinal vesicle chromatine configuration, the first polar body proportion , cumulus expansion and cleavage rate after activated, we studyed inhibitors influence on oocytes maturation cumulus expansion and development, in order to choose one kind of inhibitor which could improve oocytes maturation quality.Experiment 1: The influence of different COCs condition, different storage temperature and collection time of ovary on oocytes germinal vesical chromatin configuration, we stained COCs with Hoechst 33342 after disposal, which came from integrity or not cumulus mass cell; different ovary storage temperature (25-30℃ and 31-34℃); different collection time (Aug-Sep and Nov-Dec) ,then observed oocytes germinal vesical chromatin configuration. Experiment results showed that: integrity cumulus mass cell had significantly high GVI-GVII oocytes proportion in comparison with non-integrity cumulus mass cell(74.46% vs 57.06%, P < 0.01),while these two groups had no significant distinction in GV oocytes proportion (90.98% vs 85.44%, P>0.05) ; ovary preserved at 31-34℃ had significantly lower GVI-GVII and GV oocytes proportion than ovary preserved at 25-30℃ (45.96% vs 74.46%, P < 0.01;77.62% Vs 90.98%, P < 0.01). Under different collecting time, each meiotic stage oocytes were basically in the similar proportions, except that GVI oocytes proportion was influenced by collecting time. In conclusion, integrity or not cumulus mass cell condition and ovary storage temperature could affect oocytes germinal vesical chromatin

Related Dissertations

  1. Exoression of Resistin During Porcine Preadipocyte Differentiation and Its Infiuencing Factors,R587.1
  2. Effects of Enzymes on Metabolism of Polycyclic Aromatic Hydrocarbons in Plant,X173
  3. Studies on the Difference of PRRSV Infectious and PRRSV ADE,S858.28
  4. Genetic Variation Analysis of Porcine Reproductive and Respiratory Syndrome and Eukaryotic Expression of Pig Interferon α,S858.28
  5. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Immature Mouse,S865.13
  6. Isolationand Identification of Porcine Parvovirus and Parts of Its Biological Characteristics,S852.65
  7. Molecular Cloning, Prokaryotic Expression and Characterization of a Novel Trypsin Inhibitor in the Frog, Lepidobatrachus Laevis,Q78
  8. Study on Tetraploid of Chrysanthemum Nankingense Induction in Vitro and Its Breeding Utilization,S636.9
  9. Studies on Cytology and Molecular Cytology of Five Speceis in Genus Artemisia,S682.11
  10. Molecular Biological Characteristics of Multiple Porcine Fc Gamma RⅡB Sub-isoforms Generated by Alternative Splicing,S828
  11. The Effects of PCV2 on NF-κB Signal in Piglet’s Lymphocytes in Vitro,S858.28
  12. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  13. Study on the Expression and Bioactivities of Porcine Interleukin-2/6 Fusion Protein in Vivo and in Vitro,S858.28
  14. Detection Classical Swine Fever Virus and Porcine Circovirus Type 2 of Microarray Technique,S858.28
  15. Proliferation Characteristics of Type 2 Porcine Circovirus Strains and Antivirus Activity of Iprs and Aps on PCV2 in Vitro,S858.28
  16. The Study on Influence of Transcriptional Profiles of Porcine Peripheral Blood Lymphocytes Cultures Following Infection with Porcine Parvovirus,S858.28
  17. The Research of the Function of Near N-terminal Structral Domain of Sialic Acid and the Effect of Lps on IFN-α Generated from PAM Infected with PRRSV,S858.28
  18. Vitrification of Porcine Early Stage Embryos Reconstructed by Somatic Cell Nuclear Transfer,S828
  19. Changes in Histone H2A Variants During Mouse Oocyte Growth and Pre-Implantation Embryos,S865.13
  20. Investigation of Epidemiology of Porcine Circoviruses in JinHua of ZheJiang Province,S858.28
  21. Effects of Cumulus Cells on Maturarion in Vitro and Subsequent Developmental Competence of Porcine Oocytes,S828

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Pig
© 2012 www.DissertationTopic.Net  Mobile