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The cinnamic aldehyde promote the molecular mechanism of NIH3T3 cell proliferation and collagen synthesis

Author: ZhaoJingXia
Tutor: HuangQiFu；LiPing；LiKeLing
School: Beijing University of Traditional Chinese Medicine
Course: Chinese and Western medicine combined with the basis
Keywords: Chronic Skin Ulcer Cinnamyl Aldehyde Collagen Synthesis Proliferation
CLC: R285
Type: Master's thesis
Year: 2006
Downloads: 88
Quote: 0
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Abstract


Chronic skin ulcer（CSU） is a kind of non-healing wound because of poor repair, which can be secondary to many disease and has given a heavy burden to patients.Cutaneous wound repair can be divided into a series of overlapping phases including fibroblast proliferation and migration, granulation tissue formation incorporating re-epithelialisation and angiogenesis and finally, matrix formation and remodeling. Fibroblasts are the principal cells for repair that play a vital role throughout the entire wound healing process. Chinese medicine has preferable curative effect on CSU. According to traditional Chinese medicine theory, CSU with the differentiation of Yinzheng is usually treated with the principle of“rescuing YANG”. In all the formulas of“rescuing YANG”we have collected, we found that Cinnamomum cassia was used most widely. Cinnamyl aldehyde is the essential component of Cinnamomum cassia. The purpose of our study is to investigate the mechanism of Cinnamyl aldehyde（CA） on NIH3T3 proliferation and collagen synthesis at an angle of cell biology。1.Effects Of CA On NIH3T3 Proliferation And collagen synthesis（1） Cell proliferation was measured by MTT assay.The results indicated that the growth of NIH3T3 cells was inhibited when the concentration of CA was over 3.5×102ng/ml; the proliferation was promoted obviously when CA was between 8.8×10ng/ml and 3.5×10-1ng/ml. The promotion of CA on NIH3T3 proliferation reached the maximum at the concentration of 5.5ng/ml and the proliferation rate was 15.8%.This concentrations （5.5ng/ml） was used in the next experiments.（2） Hydroxyproline assay kit was used to detect the effects of CA on NIH3T3 collagen synthesis. The ability of collagen synthesis was inhibited when the concentration of CA was over 3.5×102ng/ml; it was elevated obviously when CA was between 8.8×10ng/ml and 8.8×10-2ng/ml. The promotion of CA on NIH3T3 collagen synthesis reached the maximum at the concentration of 5.5ng/ml and 2.2×10ng/ml.The two concentrations had no obivious difference.（3） Cell wound experiment in vitro embodies the motility and proliferation abilities of cultured cells. The effects of CA on wound healing were determined by means of time-lapse photo-images. bFGF was used to be positive control. CA and bFGF could improve the healing rate of NIH3T3 cell wounds. bFGF had better effect than CA.2.Mechanisms Of CA On NIH3T3 Proliferation And collagen synthesis（1） The effect of CA on cell cycle phase distribution of NIH3T3 cells was assessed by flow cytometry analysis. CA decreased the proportion of cells in the G0/G1 phase by 3.3% and increased the proportion in the S phase of the cell cycle by 3% after 24h treatment. The

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