Dissertation > Excellent graduate degree dissertation topics show

Effect of Esculentoside a on Apoptosis and Expression of PCNA、Caspase-3、Fas and FasL in Renal Tissue of BXSB Mice

Author: MengLingGuo
Tutor: ZhangXiangGui
School: Zunyi Medical College,
Course: Internal Medicine
Keywords: esculentoside A lupus nephritis proliferation cell nuclear antigen apoptosis Caspase-3
CLC: R285.5
Type: Master's thesis
Year: 2011
Downloads: 4
Quote: 0
Read: Download Dissertation

Abstract


Objective:To explain the mechanism of EsA effects LN and effects on the expression of PCNA、Caspase-3、Fas and FasL in renal tissue of BXSB mice.Methods:24 male BXSB mice at the age of 18 weeks were randomly divided into three groups:model group、EsA-treated group and Dexamethasone-treated group. Each group were treated,all mice were obtained urine randomly,and were killed to obtain renal tissue after 4 weeks. The following parameter was detected:1.Urine protein and urine creatine concentration;2.HE staining of renal pathology.3.The expression of PCNA and Caspase-3 were detected by in stiu hybridization in renal tissue;4.The expression of Fas and FasL proteins were detected by immunohistochemtry in renal tissue.Results:1.At last,the level of urine protein/urine creatinine was decreased in EsA-treated group and Dexamethasone-treated group (P<0.05);There is no difference between EsA-treated and Dexamethasone-treated group(p>0.05);2.After using drug,the renal pathological change of BXSB mice moderates significantly;3.The expression of PCNA mRNA in renal tissue of model group was significantly higher than it of EsA-treated group and Dexamethasone-treated group (P<0.05).There is no diference between EsA-treated group and Dexamethasone-treated group(P>0.05);4.As for,the expression of Caspase-3 mRNA、Fas and FasL in renal tissue of model group was lower than them of EsA-treated group and Dexamethasone-treated group,and there has significance in three groups(P<0.05),but there is no difference between EsA-treated group and Dexametha-sone-treated group(p>0.05).5.Correlation showed that there was no correlation between the expression of Caspase-3 and Fas in mesangial cell in three groups.Conclusion:1.Experimental doses of EsA and Dexamethasone can degrade urine protein concentration and improve renal pathology,so they can reduce renal damage of BXSB mice;2.EsA and Dexamethasone can reduce renal damage by the potenial meshanism of inhibiting renal cell proliferation and improving renal cell apoptosis of BXSB mice.3.There was no correlation between the expression of Caspase-3 and Fas of mesangial cel of BXSB mice each group.

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  3. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  4. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  5. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  6. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  7. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  8. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  9. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  10. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  11. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  12. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  13. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  14. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  15. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  16. Effects of TACI-Ig on MRL/lpr Mice and Activation of JAK1-STAT1 Signaling Pathway in Lupus Nephritis,R363
  17. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  18. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  19. The Effects of (180F-FDG on the Apoptosis of Eca-109 Esophageal Cancer Cell Line,R735.1
  20. The Signal Mechanism Underlying DON Induced Apoptosis in BHK-21 Cells,S856.9
  21. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology
© 2012 www.DissertationTopic.Net  Mobile