Dissertation > Excellent graduate degree dissertation topics show

The Experimental Investigation of Non-heart-beating Donor in the Rat Lung Transplatation

Author: LiaoDongShan
Tutor: LiaoChongXian;LiZengQi;ChenDaoZhong
School: Fujian Medical
Course: Department of Cardiothoracic Surgery
Keywords: Non-heart-beating donor Rat Lung transplantation Experimental investigation
CLC: R655.3
Type: Master's thesis
Year: 2002
Downloads: 29
Quote: 0
Read: Download Dissertation

Abstract


Background: A shortage of lung donors pool is a serious obstacle to the widespread application of lung transplantation for end-stage lung disease. The desire of enlarging the donors pool compels us to explore the feasibility of non-heart-beating donor in rat lung transplantaion.Methods: Sixty Sprague-Dawley rats were randomly divided into 3 groups that lung of the rat were harvested immediately, 30 minutes and 60 minutes after cardiac-arrest. All the donor heparinized lungs were ventilated at the moment when they were flushed with the low potassium dextran solution and then stored in the same solution at 4 for 4 hours. The recipient animal underwent left thoracotomy, and then orthotopic left lung transplatation. The right pulmonary hilum were ligated 1 hour after reperfusion.Results: All the recipients of heart-beating lungs and 30 minutes cadaver lungs survived the observation period with excellent gas exchange, whereas 4 of 10 recipients of 60 minutes cadaver lungs survived for 10 minutes after the ligation of right pulmonary hilum and 3 for 20 minutes. The microstructure, pulmonary compliance, energy metabolism and other markers show no significant difference between HBD group and NHBD-30 group.Conclusion: The adoption non-heart-beating donor can be a safe and effective method to increase the lung donor pool. Since the ischemia-reperfusion injury shows more serious with the longer warm ischemic time, there should be a definition for the warm ischemia time, such as 30 minutes for the rat.

Related Dissertations

  1. The Effect of Tanshinone ⅡA and Niacin on the Cardiovascular Function of Infant Obese Rat,R285.5
  2. Therapeutical Effects of Diammonium Glycyrrhizinate Lipid Ligand on Nonalcoholic Steatohepatitis and Its Partly Mechanisms of Action,R575.5
  3. Dynamic Effect of Cold Restraint Stress on Damage of Ultrastructure and Expression of Heat Shock Protein in Rat Tissues,S858.91
  4. Experiment Study of Ethylhexyl Phthalate (DEHP) and It’s Metabol-ites (Single-ethylhexyl Phthalate, MEHP) Influencingon Sper Matogoniain Young Male Wistar Rats,R329
  5. Study on the Cell Cycle of Liver Cells during Rat Liver Regeneration,Q253
  6. The Regulatory Role of Proliferation and Apoptosis of ERK1/2 and JNK Signaling Pathways in Eight Liver Cell Types during Rat Liver Regeneration,Q418
  7. BMP2 and VEGF165 Genes Transfected Rat Bone Marrow Mesechymal Stem Cells Simultaneity Expression,R329
  8. Effects of High-salt Dieet on Blood Pressure and the Remodeling of Carotid Arteries and the Intervention of Telmisartan in Wistar Rats,R544.1
  9. The Espression of C-reactive Protein in Rats of the Chronic Obstructive Pulmonary Disease with Diabetic,R563.9
  10. Relationship and Significance of Gene Transcription Profiles in Rat Liver Regeneration and Non-Alcoholic Fatty Liver Disease Occurrence,R575.5
  11. The Expression of Visfatin in Liver of Rats in Different Glucose Metabolic Statuses and Effects to Glucose and Energy Metabolism,R587.1
  12. The Beneficial Effects of Soy Isoflavones in Osteoporosis of Ovariectomized Rats,R580
  13. Morphological Characteristics of Brain Capillary Endothelial Cells and Neurons in a Ticellular System for Study on Neuroprotection and Neuroregeneration in Vitro,R651.1
  14. Influences of Temperature on SCF/c-kit Prolifertation Pathway and Cell Cycle of Rat Spermatogonial Cells Cultured in Vitro,R698
  15. The Mechanism of CREG Regulated Neuronal Apoptosis in Rat Intracerebral Hemorrhage,R743.34
  16. The Effects of Lead Exposure on Adult Rat Retina Neurons BACE-1 Expression and Downstream Products Aβ in the Early Period,R749.16
  17. The Experimental Research of VEGF Transfected Neural Stem Cells for the Treatment of Cerebral Palsy in the Neonatal Rats,R742.3
  18. The Effect of Maternal Pregestational Cus on the Serum CRH and COR, Brain Dopamine and 5-ht Metabolisms and Its Correlation with the Behavior of Offspring Rats,R714.2
  19. Experimental Study of the Effects of Butylphthalide on the Survival of Long Random Pattern Skin Flap in Rats,R622
  20. Igh Permeability Glucose Solution Caused Athological and Physiological Changes in Dorsal Root Anglion in Rats,R614
  21. Effect of Blood Glucose Fluctuation on Renal Pathological Change and Collagen Ⅳ Expression of Diabetic Rats,R587.1

CLC: > Medicine, health > Surgery > Of surgery > Science in the chest outside the > Bronchus and lung
© 2012 www.DissertationTopic.Net  Mobile