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Inhibition of RAW264.7 Macrophage Inflammatory Cytokines Release by Guggulsterone

Author: ChenChao
Tutor: HuangRuZhu;ZhangHuiJie
School: Fujian Normal University
Course: Biochemistry and Molecular Biology
Keywords: Guggulsterone RAW264.7 IL-1β TNF-α Signaling pathway
CLC: R285
Type: Master's thesis
Year: 2011
Downloads: 12
Quote: 0
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Abstract


Guggulsterone, a plant sterol derived from the oleogum resin from the guggul tree, Commiphora mukul, has been used in Ayurvedic medicine. It has been reported to possess a variety of pharmacological activities. In this study, RT-PCR was used to evaluate the effect of guggulsterone on RAW264.7 polarization from an alternative M2 activation state to classical Ml activation, and the secretiveness of inflammatory factor in RAW264.7 stimulated by LPS. The molecular mechanism underlying the anti-inflammatory activities of guggulsterone was also determined.The mRNA level of Mgll and Mg12 are increased by guggulsterone in RAW264.7 stimulated by IL-4. It suggests that RAW264.7 is polarized to anti-inflammatory M2 phenotype by guggulsterone. Furthermore, guggulsterone inhibits the expression of IL-lβand TNF-a in RAW264.7 stimulated by LPS. According to the further study, addition of guggulsterone inhibited the activation of NF-κB and ERK1/2, but not that of JNK and P38. It suggests that Guggulsterone may inhibit LPS-induced production of IL-lβand TNF-αvia suppression of NF-κB and ERK1/2 signaling pathway. Therefore, guggulsterone may play a role in inhibiting the inflammatory response. The research exposes the mechanism of guggulsterone on inhibiting the inflammatory response, and it may provide theoretical basis to the chronic inflammatory disease treatment by guggulsterone, including diabete, angiocardiopathy and other chronic inflammatory diseases.

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