Dissertation > Excellent graduate degree dissertation topics show

Magnesium Lithospermate B on human aortic endothelial cells

Author: ZhangZhiXin
Tutor: HaoZuo
School: Beijing University of Traditional Chinese Medicine
Course: Chinese and Western medicine combined with the basis
Keywords: Magnesium Lithospermate B human aortic endothelial cells free radical injury inflammatory factor adhesion molecule
CLC: R285
Type: Master's thesis
Year: 2006
Downloads: 179
Quote: 0
Read: Download Dissertation

Abstract


Introduction: Dan-shen root is one of traditional Chinese herbs which can promote blood circulation and to remove blood stasis. With the development of medicine, many studies also found that Dan-shen root plays an important role in the management of cardiovascular disease and cerebrovascular disorders. Dan-shen root has different kinds of chemical compositions, which have different functions respectively. The pharmacological study on the Magnesium Lithospermate B (LSA-B) has been less reported because of the complex technique of obtaining the water-dissovling extracts. The main aim of this work is to investigate the machnism of LSA-B on cardiovascular system and to provide some experimental evidence for further study.Objective: To study the effect of LSA-B on the proliferation of human aortic endothelial cells (HAECs) and the possible protectively effect on the HAEC injured by the free radical.Methods: 1) Cell culture: HAECs were purchased from Cascade Company (USA) and were cultured with the medium 200. The cells of Passage 3 to Passage 6 were used in the experiment. 2) MTT test for the evaluation of cell proliferation: the effects of different concentration of Magnesium Lithospermate B on HAEC proliferation were observed. 3) The free radical injury to HAEC: Glucose Oxidase model was used in the experiment.Results: Cell damage was observed in the high concentrations of 1mg/mL 0.5mg/mI. There is not injurious effect in the concentrations below 0.2mg/ml on the cell proliferation. An evident protective effect of Magnesium Lithospermate B on HAEC injured by the free radical was observed.Conclusions: There is a large gentle concentration range for using Magnesium Lithospermate B in the management of vascular disease. They have no toxic effect on EC proliferation, and show a protective effect on HAEC against the free radical injury.

Related Dissertations

  1. Adhesion Molecule P-selectin Expression in Gastric Cancer Peritoneal Metastasis,R735.2
  2. The Roles and Expression Patterns of VCAM-1 in Rat’s Organs with Anaphylactic Shock,R593.1
  3. Experimental study of multiple myeloma thrombosis related factors,R733.3
  4. The Protective Effect of Bone Marrow Stromal Cells of Children with Leukemia on Leukemic Cells and the Influence of Cell Adhesion Molecule VLA-4 on the Effect,R733.7
  5. The Study of Henshenluo on Expression of Proliferating Cell Nuclear Antigen (PCNA) and Intercellular Adhesion Molecule-1 (ICAM-1) of 5/6 Nephrectomy Rats,R285.5
  6. The Influence of Glycyrrhizin on the Nuclear Factor-κbp65, Tnf-α, Il-1β, IL-6 and ICAM-1 of the Severe Acute Pancreatitis in Rats,R285.5
  7. Effects of Tongxinluo on VCAM-1 and PAI-1 Expression in Atherosclerotic Rabbits,R-332
  8. The Appraisement to the Relation between Inflammation and Central Arterial Blood Pressure in Which Telmisartan Are Used to Delay Artery to Harden,R543
  9. Expression of Oxygen Free Radical and Intercellular Adhesion Molecule-1 Following Subarachnoid Hemorrhage in Rats,R743.35
  10. Carnosine Eyedrops Inhibits Early Stages of Retinal Microvascular Lesions in Diabetic Rats,R774.1
  11. Effects of Atorvastatin on the mRNA Expression of VCAM-1 and ICAM-1 in Cultured Human Umbilical Vein Endothelial Cells,R96
  12. The Effects of Three Mechanical Ventilation Strategies on Inflammatory Factors and Type Ⅱ Alveolar Epithelium Cell Morphology in Newborn Piglets with Acute Lung Injury,R563.8
  13. CO-EFFECTS OF SOY ISOFLAVONE COMPANY WITH VC &VE ON THE EXPRESSION OF ADHESION MOLECULES AND CYTOKINES IN TYPE 2 DIABETIC MELLITUS,R587.1
  14. Protective Effect and Mechanism of Cortex Moutan on Experimental Autoimmune Hepatitis to C57BL/6 Mice,R285.5
  15. Intervention Study buck clock net square spontaneously hypertensive rats inflammatory response and left ventricular remodeling,R285
  16. Qingnaoxuanqiao Dripping Pill on cerebral ischemic injury protection effect and mechanism of,R285
  17. Study on Pre-eclampsia’s Relationship with Vascular Cell Adhesion Molecule-1, Intercellular Adhesion Molecule-1 and Platelet Endothelial Cell Adhesion Molecule-1,R714.245
  18. Change and Significance of IL-18 and CD54 in Children with Congestive Heart Failure,R725.4
  19. The Mechanism and Effects of Tripotolide on T Helper-2 Cytokines and Vascular Cell Adhesion Molecule-1 of Rats with Allergic Rhinitis,R285.5
  20. The Effects of Qimu Kidney Tonifying Decoction on the CTGF and ICAM-1 Expression of Experimental Diabetic Nephropathy Rats,R285
  21. The Function of Complex Prescription Qishenjiangzhiyin in Treating Artherosclerosis and Its’ Possible Mechanism,R285

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology
© 2012 www.DissertationTopic.Net  Mobile