Dissertation > Excellent graduate degree dissertation topics show

Establishment of the Nested-Real-Time-PCR for Detection of Human Transcription Factor FOXP3mRNA Expression and Its Clinical Application in Dignosis of Rheumatoid Arthritis

Author: CaoXinGuo
Tutor: WangLiWen
School: Nanjing University of Traditional Chinese Medicine
Course: Integrative Medicine Clinical Laboratory
Keywords: FOXP3 RA Nested-Real-time-PCR CD4~+CD25~+ T cell IFN-γ/IL-4
CLC: R593.2
Type: Master's thesis
Year: 2006
Downloads: 161
Quote: 0
Read: Download Dissertation

Abstract


ObjectiveTo develop a Nested-Real-Time-PCR method for the detection of F0XP3mRNA in human peripheral blood mononuclear cells(PBMC), study the correlation of F0XP3mRNA with CD4~+CD25~+ Regulatory T cell, ratio of CD4 and CD8, Th1 and Th2, syndrome types of Traditional Chinese Medicine(TCM).MethodsRNA was isolated from PBMC and reversed transcription into cDNA as template for PCR. Design outer and inner primers by FOXP3 gene sequence. Detect F0XP3mRNA gene expression level in Nested-Real-Time-PCR using Taqman probe, detect number of CD4~+CD25~+ Regulatory T cell using flow cytometry and detect IFN- γ and IL-4 in plasma using Enzyme-Linked Immune Sorbent Assay (ELISA)at the same time.ResultsThe production of PCR was proved as specific FOXP3 segment by sequence assay. Total mean Ct(Cycle Threshold) in 6 tests was 14.66. The mean CV (coefficient of variation) was 5.81%. The standard curve showed a fine linear relationship between Ct and template concentration, and the correlation coefficient was 0.999. The sensitivity was 100 times higher than normal real-time PCR by reach 10° copy. FOXP3mRNA expression, number of CD4~+CD25~+ Regulatory T cell, ratio of CD4 and CD8(CD4/CD8), ratio of IFN-γ and IL-4(IFN-γ/IL-4) were detected in 31 Rheumatoid Arthritis(RA) patients and 10 health person. FOXP3mRNA expression, number of CD4~+CD25~+ Regulatory T cell, CD4/CD8 of RA patients were higher than health peason(P<0.01). The FOXP3mRNA expression and the number of CD4~+ CD25~+ Regulatory T cell were positive correlation, P=0.004. The 31 patients belongs

Related Dissertations

  1. The Induction of Proteins ESAT-6 and Ag85B Secreted by Mycobacterium Tuberculosis on FoxP3mRNA in Peripheral Blood,R392
  2. Experimental Research on Stressing Performances of Recycled Concrete Simply-supported Beams,TU375.1
  3. Sirolimus Induces Rat Na(?)ve CD4~+ T Cells to Regulatory T Cells and Its Application,R965
  4. The Study on Th1/Th2 Drift and ShuXueTong Injection’s Intervention in UA Patients,R259
  5. Expression and Significance of CCR6, CCR7 and CD4~+CD25~+Foxp3~+ Regulatory T Cells in Laryngeal Squamous Cell Carcinoma and Its Progression,R739.65
  6. Association Research between Polymorphism of IFN-γ and IL-10, Environmental Risk Factors, and Susceptibility to Esophageal Cancer,R735.1
  7. Study the Relationship of CD4~+CD25~+Foxp3~+ Regulatory T Cells and the Clinical Stage and Chemotherapy of Lung Cancer,R734.2
  8. Preliminary Study on Injury Mechanism of Cellular Immunity of Latent Autoimmune Diabetes in Adult,R587.1
  9. Level of T-bet, GATA3 and FOXP3 mRNA Expression on the Peripheral Blood Mononuclear Cells of Patients with Systemic Lupus Erythematosus and Its Significance,R593.241
  10. Low Responder T Cell Susceptivity to the Suppressive Function of Regulatory T Cell in Patients with Dilated Cardiomyopathy,R542.2
  11. Query by Singing and Humming System,TP391.3
  12. Expression and Significance of FOXP3 in Pityriasis Rosea, Lichen Planus, Large-Plaque Parapsoriasis and Mycosis Fungoides,R758.63
  13. Study on the Role of Th17/Treg Balance in Normal Pregnancy and Preeclampsia,R714.245
  14. The Influence of H. Pylori on Tim-3 in Lymphocytes and Gastric Muconsa of Mice and Th Immune Response and the Correlation between Them,R57
  15. Effect of Oral Atorvastation on Inflammatory Factor in the Infarct-related Coronary Artery in Patients with ST-segment Elevated Myocardial Infarction Prior to Primary Percutaneous Coronary Intervention,R542.22
  16. The Effects of GA and CCC on Main Agronomic Traits, Physiological Indexes and Steviol Glycosides Yields of 4 Different Stevia Rebaudiana Lines,S566.9
  17. Mechanism Study of Anti-tumor Effect of Triptolide in B16-F10 Tumor Bearing Mice Based on Treg,R285.5
  18. The role of Th17/Treg Unbalance in the Immunologic Pathogenesis of EAE,R744.51
  19. Preliminary Studies on Transplantation of Umbilical Cord Mesenchymal Stem Cell to Treat Liver Cirrhosis,R575.2
  20. The Extract of Shiraia Bambusicola and Its Effects on the TNF-α Pathway in Rheumatoid Arthritis,R285.5
  21. The Study of the Percutaneous Absorption of the Flexible Nanonipsomes of Diclofenac Sodium for External Application and the Influence on the Analgesic and Antiinflammatory Effect of Rats with Rheumatoid Arthritis (RA),R94

CLC: > Medicine, health > Internal Medicine > Systemic disease > Autoimmune diseases > Autoimmune diseases, connective tissue disease
© 2012 www.DissertationTopic.Net  Mobile