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The Direct Electrochemical Behavior of Cytochrome c and Its Modified Electrode Preparation and Application

Author: LiuYunChun
Tutor: YangZhouSheng
School: Anhui Normal University
Course: Analytical Chemistry
Keywords: Cytochrome c Direct electrochemical behavior DNA L-cysteine Electrocatalysis NO
CLC: Q559.9
Type: Master's thesis
Year: 2006
Downloads: 240
Quote: 0
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Abstract


Cytochrome c is an integral part of the organisms in the electron transfer chain , located in the mitochondrial inner membrane , in the oxidation-reduction reaction of the living body is considered to be a typical example of the biological electrochemical redox reaction , therefore , cytochrome c electrical the chemical properties of aroused widespread concern . This paper studies the direct electrochemical behavior of cytochrome c and its modified electrode application to carry out the experimental work . The paper is divided into two parts : the first part , a simple method for preparation of DNA modified glassy carbon electrode modified glassy carbon electrode DNA can promote cytochrome c electron transfer on the electrode , we get the cytochrome c direct electrochemical behavior , and DNA - modified electrodes applied to the quantitative detection of cytochrome c . The second part discusses the preparation and application of the cytochrome c modified electrode . We chose DNA and L-cysteine ??cytochrome c / DNA modified glassy carbon electrode and cytochrome c/L- cysteine ??modified gold electrodes were prepared as a coupling agent . Modified electrode surface , cytochrome c can maintain good electrochemical activity . Cytochrome c / DNA modified glassy carbon electrode in acetate buffer (pH 5.0) for NO showed good Electrocatalytic reduction phenomenon , when the NO concentration of 4.0 × 10 -6 ~ 5.0 × 10 -5 < / sup > M range between peak current and the concentration of NO into a linear relationship , the correlation coefficient was 0.996 , the detection limit is 1.0 × 10 -6 < / sup> M . However , in a PBS solution ( pH 7.0 ) , the the cell pigment c/L- cysteine ??modified gold electrode showed good electrocatalytic oxidation characteristics of NO , NO concentration in of 7.0 × -7 < / sup> ~ 1.0 × 10 -5 M between the peak current and the concentration of NO into a linear relationship , the correlation coefficient was 0.996 , and the detection limit is 1.0 × 10 -7 M.

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CLC: > Biological Sciences > Biochemistry > Enzymes > Synthase > Cytochrome system
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