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Haitanensis is a high nutritional value of algae , in Fujian province has a long history of cultivation . Analysis using molecular biology techniques haitanensis germplasm genetic diversity, find related molecular markers that can guide cultivation practice , accelerate the breeding process . The first part of this experiment was constructed small fragments haitanensis genomic library was screened from about 1500 clones in 215 positive clones were screened to four microsatellite loci . Choose one of the two microsatellite loci ATN4 and GAN5 and from GeneBank Gracilaria in search of three microsatellite loci AJ512812, AJ512806 and AJ512804 for nine seaweed samples (LYG, RD, CS, GL1, DXY, BBD, JJS, NH , and LSD) for PCR amplification . Five microsatellite loci were detected in 31 DNA bands , which strips most sites are AJ512812, there are 12 ; loci strip is at least AJ512804, only a band of other loci amplified bands between 4 to 8 . In all there are 31 with 26 polymorphic species , accounting for 83.9% of the total number of bands . According to the results of micro- satellite test , using an average chain class method (Between groups linkage) between each sample Nei's genetic similarity coefficient cluster analysis . Analysis showed that wild haitanensis sample NH, JJS and LSD genetic similarity coefficient between the highest, reaching 96.8% ; haitanensis genetic similarity between each sample coefficient of 58.1% to 96.8 %, and the different sea areas , cultivated and wild samples Samples there is a big difference between genetic . This indicates haitanensis rich genetic diversity , breeding will haitanensis basis . As can be seen from the dendrogram , 9 seaweed samples copolymerized into two categories, haitanensis sample NH, JJS and LSD first clustered into one group , and then get the other four kinds haitanensis samples gathered samples LYG and Porphyra RD first got together , and the final sample haitanensis together . Two different sea farming haitanensis CS and GL1 is even greater than the genetic distance between the altar and the laver laver between genetic distance instructions will result in different geographical environment seaweed in genetically quite different, sometimes even greater than interspecific the genetic distance. Five microsatellite loci in nine seaweed samples were amplified six specific bands . Yezoensis
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