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Two-line hybrid rice is rice heterosis utilization of a major innovation, light and temperature sensitive genic male sterile line is the basis of two-line, two-line key to success lies in whether bred for large-scale production of dual-use applications genic male sterile. Over the years, our breeders are dedicated to two-line hybrid rice research, breeding a number of advantages of a strong two-line hybrid rice, but there have been many urgent problem, breeding low critical temperature, excellent comprehensive properties , with strong dual-use nuclear male sterile line is still two-line focus of the work. This study is based on two light TGMS 201S M113S and China as the research object, and Pei'ai 64S as control the use of different seeding methods and continuous inspection pollen fertility selfing rate changes, research CMS Fertility conversion characteristics; simultaneously with these two CMS hybrids with six conventional varieties investigated six combinations of F 1 , F 2 , BC 1 on behalf of the pollen fertility, selfing, three indicators under natural seed fertility, study their genetic expression; while the indica and japonica CMS attributes, infertility gene is also studied allelic ; also to the M113S and China 201S as the female parent, respectively, with more than 80 combined hybrid, main agronomic traits of F1 examine, analyze heterosis, screening strong advantage combinations. The main findings are as follows: 1. The conversion characteristics of sterile alteration studies have shown that different light and temperature sensitive genic male sterile sensitive to temperature and different light, temperature and light on different CMS coefficient of determination is also different. M113S fertility sensitive stage in its conversion before heading 13-25 days, China 201S fertility sensitive stage in its conversion before heading 14-25 days Peiai 64S fertility sensitive stage in its conversion before heading 13 -22 days. Wuhan, Lingshui fertility investigation showed that Chinese 201S is the critical temperature is lower, more stable sterility CMS. 2. Artificial climate chamber identification results show, M113S is the starting point lower temperature (≤ 23.5 ℃) light and temperature sensitive genic male sterile. 3. F 1 Fertility analysis showed that the six combinations of F 1 on behalf of the three indicators of fertility performance under all fertility restoration, indicating sterility CMS is controlled by a recessive gene. F 2 -generation fertility analysis showed that the six combinations of F 2 -generation fertility segregation bimodal or multimodal distribution of peak fertility, infertility peaks location, high and low, indicating genic male sterility gene expression in different degrees. Divided according to the conventional method of fertility, six combinations of F 2 and BC 1 -generation fertility segregation chi-square test showed that the combination of F 2 obey 15:1 or 13:3 (fertile plants: sterile plants) separation, BC 1 obey 3:1 (fertile plants: sterile plants) separation, indicating M113S and Chinese 201S infertility traits are controlled by two pairs of genes. 4. Sterility gene allelic analysis shows, M113S, China 201S, 64S twenty-two Pei'ai hybrid F 1 -generation fertility performance of infertility, and M23S, M103S hybrid F 1 on behalf of pollen sterility were 95.59%, 91.22%, 95.42%, selfing rates were 41.72%, 47.41%, 41.55%, natural seed rates were 75.58%, 80.52%, 68.67%, indicating M113S, China 201S, the three CMS Pei'ai 64S infertility gene allele, and M23S, M103S infertility gene allelic.
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