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Social Control on Chromosome Number Variation by Cell Apoptosis Pathway in Citrus Callus

Author: ZhouZhou
Tutor: DengXiuXin
School: Huazhong Agricultural University
Course: Pomology
Keywords: Citrus Callus Tetraploid cells Co-culture Apoptosis Isolated from cultured
CLC: S666
Type: Master's thesis
Year: 2006
Downloads: 122
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Abstract

Somatic chromosome number variation phenomenon prevalent in plant tissue culture , which is due to abnormal mitosis caused . Studies have shown that some plants under in vitro culture conditions , its chromosomal polyploidy variation often extend over time increase . However , citrus callus subculture 10 years still has a strong ability to embryogenesis and chromosome ploidy composition has remained relatively stable . The predecessors while continuously generated speculated the diploid callus tetraploid cells to apoptosis of the higher frequency mode is eliminated, so as to maintain the stability of the tetraploid cell ratio . To test this hypothesis , the paper will be labeled with a green fluorescent National One diploid cells and low-density embedded culture resulting tetraploid cells mixed suspension culture inbred tetraploid cells embedded sections were picked up and found that some tetraploid cells showed typical features of apoptosis . Change the culture conditions , statistics apoptosis rate co-cultivation time and the proportion of the relationship . The results showed that cultured for 2-7 days , mixed culture of tetraploid apoptosis rate when cultured alone significant difference ; sample tetraploid cells than diploid , tetraploid showed higher withered mortality ; apoptosis rate increased with increasing time , two days of apoptosis rate increased significantly . In situ end labeling analysis showed equal mixture of co-cultured 10 days of apoptosis rate of 36.9% , higher than the morphology count results , because of DNA damage in early morphological changes . In order to investigate the interaction of cells of different ploidy ways to design the pore size of 0.22μm membrane isolation culture device , no direct contact between cells , slice count statistical results showed that the co-cultured for 4 days , the tetraploid apoptosis rate alone culture when compared to the difference was not significant , which proves that in this mode , the contact between the cell groups is the way of interaction .

Social Control on Chromosome Number Variation by Cell Apoptosis Pathway in Citrus Callus

Abstract

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