Dissertation > Excellent graduate degree dissertation topics show

Agrobacterium Tumefaciens-mediated Transformation of MdSPDS1 and HAL2 Genes in Citrus

Author: ChenChuanWu
Tutor: LiuJiHong
School: Huazhong Agricultural University
Course: Pomology
Keywords: Citrus Agrobacterium tumefaciens Genetic Transformation Salt MdSPDSI gene HAL2 gene
CLC: S666
Type: Master's thesis
Year: 2006
Downloads: 140
Quote: 2
Read: Download Dissertation

Abstract


Citrus is one of the world's most important fruit, its area and production ranks first in all types of fruit, occupies an important position in people's daily life. Citrus industry there are still many problems, such as pests and diseases and natural disasters cause serious harm, maturity is too concentrated, the variety structure is irrational. Worldwide, about 20% of arable land and more than half of the irrigated fields by salinization, and this effect in increasing our country there are about 5 million acres of saline land. Citrus on the long chapter of the embryo and nucellus Polyembryony genetic highly heterozygous and parthenocarpic salt improved varieties using conventional breeding methods is limited, affecting the breeding work carried out. Agrobacterium-mediated transgenic approaches breakthrough, as well as various types of plants to environmental stresses gene cloning, genetic engineering of citrus resilience laid the foundation. In this study, fine citrus varieties of sweet orange callus and epicotyl explants, Agrobacterium tumefaciens-mediated transformation MdSPDS1 and the HAL2 gene expectations salt germplasm. The main results are as follows: 1 kanamycin Peng navel orange and Orange Frost embryogenic callus. Determine the in citrus transformation experiments, the filters the resistant calli Suitable concentration of kanamycin for 50 mg / L. On Fruit and Jincheng sweet orange the epicotyl regeneration system were studied. The results show that: the regeneration of BA on the hypocotyl, when the BA concentration of 1.0mg / L, the highest reproduction frequency; the hypocotyl level ponkan and vertically the most suitable regeneration medium were: MT 1.0mg / L BA of 0.5 mg / L IAA and MT 1.0mg/LBA 1.0mg / L IAA; the hypocotyl horizontal Jincheng sweet orange on the most suitable regeneration medium: MT 1.0mg/LBA 0.5mg / L IAA. Affecting Peng navel orange callus conversion efficiency factor conducted a preliminary optimization experiments show that: when the infection time 15-20min, the infection concentration OD 600 value of 0.8, a total number of training days 3d, the highest conversion efficiency. 4 MdSPDS1 gene and HAL2 gene conversion Peng navel orange resistant callus after repeated subculture, normal growth, via the the npt Ⅱ and 35S-MdSPDS1, NPT II, ??and the HAL2 primer PCR amplification analysis and identification proved to be genetically modified product. 5 will be the MdSPDS1 gene into Ban Na navel orange transgenic lines salt stress (100 mM), results showed that the fresh weight of callus proliferation of transgenic lines is higher than the control, the conductivity is lower than in the control, confirmed the salinity tolerance of transgenic lines higher than that of the control. The HAL2 the gene transformation Peng navel orange transgenic lines will be 6 to salt stress (100 mM), results showed that the fresh weight of callus proliferation of transgenic lines compared with the control, lower conductivity than the control confirmed the salt tolerance of the transgenic lines higher than that of the control.

Related Dissertations

  1. Cloning and Expression of CHS and CHI Genes and Their Regulation on the Accumulation of Flavonoids in ’Cara Cara’ Navel Orange (Citrus Sinensis Osbeck) and ’Guoqing NO.4’ Satsuma Mandarin (Citrus Unshiu Marcow),S666.4
  2. Effects of CRZ-1 on Growth of Wheat under Salt Stress, Chlorophyllase and Yield,S512.1
  3. Improvement of the Resistance of Nicotiana Tabaccum to Virus by RNAi and Initial Establishment of Genetic Transformation System of L.regale,S435.72
  4. Transformation of RdreBlBI Gene Drived by Rd29A in ’Benihope’ Strawberry,S668.4
  5. Citrus Image Segmentation Based on Genetic Algorithm,TP391.41
  6. The Effects of Overexpression of OsSsr1 on Stress Tolerance in Tobacco,S572
  7. Preliminary Study on Salt-Tolerant Mechanism of the New Pennisetum Purpereum Schumach Variety-Sumu No.2,S543.9
  8. Biological Effect of Rare Earth Element and Genetic Transformation on Arnebia Euchroma(Royle) Johnst.Cell,S567.239
  9. Ropgef Family Gene in Response to Abiotic Stresses and Expression at Different Tissues of Arabidopsis,Q945.78
  10. A Research on the Modernization Project of Jiangsu Edible Salt Logistics,F259.2
  11. Cloning 1Dx5 Gene from the Xinjiang Wheat Variety and Constructing Its Expression Vector,S512.1
  12. Functional Characterization of Wheat Na~+ /H~+ Antiporter TaNHX2 and Study of TaNHX2 C- Terminus Domain,S512.1
  13. The Genetic Transformation and Functional Analysis of GmFtsH,S565.1
  14. Mechanisms of Alleviating Effect of Seed Soaking with Soybean Isoflavones on Glycine Max, Salt-born Glycine Soja and Their Hybrid Seedlings under Salt Stress,S565.1
  15. Cloning of Proteinase Inhibitor Gene CYS and API from Wild Potatoes and Preliminary Study of Potato and Tobacco Transformation with Spcys,S532
  16. Construction of Expression Vector and Genetic Transformation in Phalaenopsis of Phalaenopsis Floral Meristem Identity Gene LFY,S682.31
  17. Study on Transformation of Cut Chrysanthmemum with DdICE1 Gene,S682.11
  18. Study on Agrobacterium-Mediated Transformation of ’Benihonpe’ Strawberry with ALA Synthase Gene,S668.4
  19. AttM Transgenic Colored Calla Lily and Antibodies Preparation of Ahl-Degrading Enzyme AttM,S682.264
  20. Effect of Modes of Ridge and Nutrient Solution Irrigation on Cucumber Cultivation and Transport of Water and Salt in Soil,S642.2
  21. Preliminary Study on the Regeneration System and the Reporter Gene GFP Instantaneous Expression of Soft-seed Pomegranate Variety ’Tunisia’,S665.4

CLC: > Agricultural Sciences > Gardening > Fruit trees gardening > Citrus
© 2012 www.DissertationTopic.Net  Mobile