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Pumpkin (Cucurbita spp) is an important horticultural plants, pumpkin (Cucurbita moschata) breeding, has formed a rich variety of resources. The study of the genetic diversity of pumpkin germplasm resources, their germplasm resource collection, preservation, identification, innovation and rational utilization. Molecular markers reflect genetic differences between the germplasm directly from the DNA level, is the study of plant genetic diversity effective tool. In this study, using a combination of morphological markers and RAPD markers using hierarchical clustering analysis, principal component analysis, principal coordinates analysis of 32 copies of the genetic diversity of the pumpkin germplasm. The main results are as follows: 1. Pumpkin morphological traits observed standard morphological characters genetic diversity parameters statistical results showed that the coefficient of variation of 50 traits (8.43% to 101.22%), the performance of the rich diversity. The prominent stem bristle, tender the melon and old melon skin color markings shape, markings, color, commodities tender melon longitudinal diameter, tender melon cross-section shape, old melon fruit weight traits (35% to 60%) . The coefficient of variation is melon shaped traits (101.22%). 2. Obtained from the principal component analysis of morphological characters, the first three principal components of the cumulative variance contribution rate of 79.70%. First principal component variance contribution rate of 68.53 percent, it includes traits eigenvalue difference, summarizes the stems, leaves, flowers, fruit, seeds and all kinds of traits, various traits showed extensive morphological diversity. 3. Morphological traits clustering, 32 were similar coefficient of 0.29 based on leaf color, leaf margin, leaf hairs, foliar leukoplakia, melon-shaped characters can be divided into branches and main stem color, stem bristle two groups. Which material II 5Bb 159 II 5Bb 233 similarity coefficient of 0.70. Which the first groups Ⅱ 5Bb 188, Ⅱ 5Bb 189 Ⅱ 5Bb 222, Ⅱ 5Bb 201 the Ⅱ 5Bb 206 poly close; 2nd group the Ⅱ 5Bb 200 to Ⅱ 5Bb207 the Ⅱ 5Bb 230 208 Ⅱ 5Bb, poly close. Its various traits are similarities. 4.100 random primers screened, 12 primers used for RAPD markers, with 79 in the 112 with amplified polymorphism polymorphic with a ratio of 70.5%. RAPD 0.53 similarity coefficient analysis of the tested materials can be divided into two groups. The melon shaped as a long cylindrical, long pear-shaped and pear-shaped Juzaiyikuai of. 5. Comprehensive two markers system analysis results are from China pumpkin, but the correlation coefficient of intraspecific differences, the performance of the rich diversity. And morphological traits clustering RAPD cluster is less consistent.
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