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Molecular Ecology and Functions of Yeasts in Different Wasterwater Treatment Systems

Author: WangZhe
Tutor: YangQingXiang
School: Henan Normal
Course: Microbiology
Keywords: Yeast Molecular ecology Sewage treatment system PCR-DGGE
CLC: X703
Type: Master's thesis
Year: 2010
Downloads: 150
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Abstract


In this study, the two means of PCR-DGGE and traditional isolation and culture fermentation wastewater, urban wastewater and papermaking wastewater three different sewage treatment system processing unit between the yeast community characteristics and species distribution characteristics, and by isolates kinds of some ecological functions related activity detection of different sewage treatment system in yeast. The specific research include the following aspects: (1) by PCR-DGGE and bar with a plastic cutting sequencing compared richness and composition of different sewage treatment system in the yeast community diversity differences. The results showed that the yeast community diversity in the sewage treatment system is quite rich, community composition is more complex, the yeast population distribution is closely related to the water quality characteristics of each system. Between different processing systems, the advantage yeast populations richness and composition differences. The yeast population advantage in the fermentation wastewater treatment system 9 are 12 kinds (Candida sorbophila, Candida tropicalis, Dipodascus capitatus Filobasidium uniguttulatum, Kluyveromyces marxianus, Issatchenkia occidentalis, / the Pichia kudriavzevii, Saccharomyces cerevisiae, and Debaryomyces sp. Geotrichum SP. or Galactomyces SP, Pichia sp, Trichosporon sp.) and possibly a new species. Dominant populations of urban sewage treatment system of Candida sorbophila Filobasidium uniguttulatum, Kluyveromyces marxianus, Saccharomyces cerevisiae yeast Deba case (Debaryomyces sp.), Trichosporon yeast of the genus (Trichosporon sp.) Two and a possible The new species. Papermaking wastewater treatment system in the yeast community structure is relatively simple, its dominant populations genus Candida tropicalis and Deba yeast (Debaryomyces sp.) Of one kind. (2) 153 yeasts were isolated from three sewage treatment system, the classification and identification of these strains by molecular biology methods. The results showed that the isolates were identified to 17 genera, 42 kinds, contains 39 different ITS-RFLP digested type. Single strain distribution showed that yeast species different advantages of the sewage treatment system. The dominant species in the wastewater treatment system of fermentation the Rhodotorula mucilaginosa; municipal wastewater treatment plant dominant species: Candida parapsilosis, Candida tropicalis, Debaryomyces hansenii of Rhodotorula mucilaginosa and Trichosporon montevideense; papermaking wastewater treatment system, the dominant species: Candida allociferrii, Pichia guilliermondii and Stephanoascus ciferrii. The distribution of the non-dominant yeast having a system-specific. The same yeast species can be distributed in two different wastewater treatment system at the same time. (3) compare the traditional isolation and culture methods and DGGE method to study the information obtained by the ecological distribution of yeast. The results show that, in biological wastewater treatment systems, ascus class yeast dominant groups, including Candida sp. Dominant, Candida tropicalis is the dominant species. Burden yeast Trichosporon sp. Dominant groups. The highest abundance of yeast fermentation wastewater treatment system, followed by the urban sewage treatment systems, and finally papermaking wastewater treatment system. Only Debaryomyces the SP (isolated and cultured as Debaryomyces hansenii, DGGE sequence analysis not identified to the species) can also be distributed in the three processing system. The yeast can be obtained by the two methods are: Candida sorbophila, Candida tropicalis, Filobasidium uniguttulatum, Issatchenkia orientali in / the Pichia kudriavzevii, Saccharomyces cerevisiae and Trichosporon montevideense. The strains Kluyveromyces marxianus only by the DGGE method detects. Strain Cryptococcus sp. And Rhodotorula sp. Only get pure cultures, is not detected by DGGE method. (4) the yeast strain obtained amylase, lipase, protease, manganese dependent peroxidase enzyme (MNP), lignin peroxidase (LiP) and laccase (Lac) producing ability. The results showed that all strains can not produce amylase, lipase can produce: Candida parapsilosis, Dipodascus ovetensis Filobasidium uniguttulatum and Geotrichum klebahnii; can produce proteases: Cryptococcus albidus, Pichia membranifaciens, Pichia spartinae and Rhodotorula mucilaginosa; can simultaneously produce lipase and a protease: the the the of Candida palmioleophila, Candida tropicalis, Trichosporon montevideense and Yarrowia lipolytica,. 43 includes eight genera, 17 kinds of yeast capable of generating MnP in the presence of a reactive red M-3BE detected, most of which are from Candida sp, the Pichia sp. Rhodotorula SP. Bacteria Candida boidinii, Candida ghanaensis, Candida parapsilosis and the Candida zeylanoides produce LiP. All strains can produce Lac. (5) dye decolorization ability of yeast strains. The results show that, The 139 yeast within 12 ~ 72 h to varying degrees of bleaching of the dye of Reactive Red M-3BE medium, accounting for 91% of the isolates. Which 10 yeast cultured for 12 h fast decolorization decolorization rate of more than 90%, and another 38 yeast rapid decolorization in 24 h, the decoloration rate from 81.28% to 99.82%.

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CLC: > Environmental science, safety science > Processing and comprehensive utilization of waste > General issues > Wastewater treatment and utilization
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