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Chromatography-Mass Spectrometry Analysis on the Chemical Constituents of Several Herbs

Author: GuanYanLi
Tutor: DingLiSheng；ZhouYan
School: Chengdu Institute of Biology
Course: Medicinal Chemistry
Keywords: Qiang-huo (Notopterygium incisum and Notopterygium forbesii ) Chrysanthemum species Meconopsis integrifolia essential oil fingerprint flavone compounds HPLC HPLC-MS GC-MS traditional Tibetan medicine
CLC: R284.1
Type: Master's thesis
Year: 2006
Downloads: 495
Quote: 3
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Abstract


This dissertation consists of four parts. The first part reports studies on the fingerprint of Notopterygium incisum and N. forbesii by HPLC-PDA-MSn, and on the constituents of essential oil by GC-MS. The second part elaborates the chemical constituents of Chrysanthemum L. by LC-MS and GC-MS analysis. The third part reports the chemical compositions of the essential oil from the different parts of Meconopsis integrifolia. The fourth part reviews on the progress of the studies on the chemical constituents in Tibetan medicines.The first chapter is about HPLC analysis of a traditional Chinese herbal medicine Qiang-huo (Notopterygium incisum and N. forbesii ). Firstly, based on analyzing and contrasting the relative retention time and relative paek area in chromatographic fingerprint, the HPLC chromatographic fingerprint of Notopterygium incisum was established, which can used as a scientific basement for the quality evalution of this herb. Secondly, quantitative analysis were performed on the main chemical constituents of Notopterygium incisum and N. forbesii including nodakenin, nodakenetin, 6’-O-trans-feruloylnodakenin, p-hydroxypenethylanisate, notopterol and isoimperatorin. The results indicated that the contents were variable related to different growth regions. Lastly, the essential oil of Notopterygium incisum collected in different harvest times is analyzed by GC-MS.The second chapter is about HPLC-MS and GC-MS analysis of several species

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical > Chemical analysis and identification
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