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Effects of Multiwall Carbon Nanotube and Multiwall Carbon Nano-onion on the Protein Expression Profiles of Human Skin Fibroblasts

Author: ZhangPeng
Tutor: YangJun
School: Zhejiang University
Course: Toxicology
Keywords: nanotoxicology multiwall carbon nanotube proteomics multiwall carbon nano-onion
CLC: TB383.1
Type: Master's thesis
Year: 2009
Downloads: 23
Quote: 0
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Abstract


Objectives: To investigate the effects of multiwall carbon nanotube （MWCNT） andmultiwall carbon nano-onion （MWCNO） on the protein expression profiles of human skin fibroblasts （HSF） using proteomic techniques.Methods: Protein expression profiles were analyzed using 2 dimensional gelelectrophoresis, and differentially expressed proteins were identified by Matrix-assisted lasor desorption ionization-Time of Flight mass spectrometry （MALDI - TOF - MS）. The expression ofβ-actin was evaluated by immunofluorescent microscopy.Results: After low concentrations (0.06 mg·L-1 MWCNT and 0.6 mg·L-1 MWCNO)and high concentrations (0.6 mg·L-1 MWCNT and 6 mg·L-1 MWCNO) treatment for 24 h, the expression levels of 22 proteins were changed. Among them, 10 proteins, includingβ-actin, were identified. Immunofluorescent microscopy also revealed the expression changes ofβ-actin.Conclusion: MWCNT and MWCNO can affect cellular protein expression. Proteomic technique can be applied in the study of nanotoxicology, which would provide useful information for the further study of molecular mechanisms.

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