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Electrochemical Sensor and Its Application in the Production of the Laccase from Penicillium Simplicissimum Isolated from Composting

Author: ZhouZuoZuo
Tutor: ZengGuangMing；LiuJianZuo
School: Hunan University
Course: Environmental Science
Keywords: Electrochemical sensors Compost Jane Penicillium Laccase Liquid fermentation Multi-walled carbon nanotubes
CLC: TP212
Type: Master's thesis
Year: 2008
Downloads: 8
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Abstract


The composting has been regarded as a very effective approach is to dispose a solid industrial and agricultural waste, biological play an important role in this process. Electrochemical sensors are electrochemical devices based on biochemical reactions, signal transduction, and has a convenient, time-saving, high precision, has become a new detection technology in the field of environmental monitoring. Electrochemical biosensor efficiency and specificity characteristics, electrochemical sensor research focus, the key technology is its built laccase modified electrode based on multi-walled carbon nanotubes (CNTs) to create an electrical signal The larger micro-environment. The present study aims to improve the of Penicillium simplicissimum Penicillium simplicissimum laccase compost isolated yield, the electrochemical reaction of the laccase, characterization and in the amplification effect of the CNTs modified study. Completed the following work: Jane Penicillium Penicillium simplicissimum produced the Laccase the liquid fermentation conditions were studied, and results show that the shake flask cultures laccase production medium composition: of maltose 2g / L, peptone 1.2 g / L, The volume fraction of the large number of elements 100 mL / L, the volume fraction of trace elements, 25 mL / L, VB 1 quality of 100μg, Tween 80 concentration of 0.05%, containing 10 7 conidia / mL culture medium thermostat of 30 ℃ oscillation (150r/min) culture 8d, the laccase production amounted to 12.44 U / L. Initial pH 3.5 of the culture medium are most conducive to the generation of laccase, a certain concentration of Cu 2 is conducive to enzyme activity to improve the most suitable concentration of 60μmol / L. An electrochemical sensor detects this experiment developed into the separated activity in compost Penicillium simplicissimum Penicillium simplicissimum arising laccase. The sensor is based on a multi-walled carbon nanotubes (CNTs) modified glassy carbon electrode. Using carbon nanotubes to join the detection system can greatly improve the electrochemical signal analysis, higher sensitivity, selectivity and speed than conventional spectrophotometry. The study found that the optimum pH value of the electrolytic system to 5.6. The results show that: having a good linear relationship between the current and spectrophotometric assay measured the concentration of the laccase, the slope of the current detected by the current timing method shown by the correlation coefficient was 0.9835. Therefore, the electrochemical sensor for rapid detection of compost Jane Penicillium the Penicillium simplicissimum arising Laccase Activity. In addition, it adopted according to the correlation between the laccase activity and biomass to and Penicilliumsimplicissimum rapid quantitative analysis has a potential possibility.

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