Dissertation > Excellent graduate degree dissertation topics show

The Studies of Gluconobacter Melanogenus on the High Concentration Sorbitol Conversion

Author: QinQin
Tutor: ZhongChongBin；XuHui
School: Northeastern University
Course: Biochemical Engineering
Keywords: Gluconobacter melanogenus sorbitol fermentation sorbose
CLC: TQ920.6
Type: Master's thesis
Year: 2008
Downloads: 29
Quote: 1
Read: Download Dissertation

Abstract


In vitamin C fermentation industry, the important step is the conversion of L-sorbose from D-sorbitol by microorganism. As the oxidation rate of batch fermentation decreased obviously with the high initial sorbitol concentration, L-sorbitol yield decrease. In the paper, the substance concentration was studied in the vitamin C fermentation technique of Gluconobacter melanogenu and the fermentiton conditions of microorganism in 35%sorbitol liquid medium were investigated.The results showed that the higher substance concentration was, the more obvious inhibition effects were. High concentration of substance and products inhibited the production of sorbose at the initial sorbitol concentration of 30 and 35 percent, however, the microorganism could defend the inhibite effects. So the Gluconobacter melanogenus had a high endurance to the concentration sorbitol and sorbose and a rate of convertion fromD-sorbitol to L-sorbose.The fermentation results showed the growth of Gluconobacter melanogenus was affected by both pH and the concentration of sorbitol while the production of sorbose was influenced by pH and growth state of microorganism in 35%sorbitol liquid medium. The more active the microorganism grew, the slower the production speed of sorbose was.The factors affecting growth metabolism, such as volume of Erlenmeyer flask, pH, inoculum and the concentration of calcium carbonate were investigated. The results showed that the optimal culture condition were 180 mL of medium in a 750 mL Erlenmeyer flask and pH ranged 5.0-6.0 after inoculation and not below 4.1 in fermentation terminal. The optimal inoculation was the medium in fermentation terminal. The optimal concentration of calcium carbonate was 0.7 percent which could regulate pH and accelerate the production of sorbose.The optimized fermentation results showed the inhibited effects of high concentration sorbose were alleviated. The concentration of sorbose reached 362 mg·mL-1 and the production velocity of sorbose was 12.1 mg·mL-1·h-1 in 35% sorbitol liquid medium at 30 h after inoculation.

Related Dissertations

  1. Haihong Fruit Vinegar Processing Technology,TS264.22
  2. Penicillium Expansum TS414 Lipase in Pichia Pastori: Expression, Purification and Enantioselective Esterification of (R, S)-naproxen,Q814
  3. Studies on Fermentation Optimization, Purification and Enzyme Characteristics of Lipase from Aspergillus Oryzae FS-1,TQ925.6
  4. Study of Mutation and Breeding of Griseofulvin Produccing Strain FS80,TQ927
  5. Optimization of Fermentation Conditions, Purification, Cloning and Expression of a Cold-active Lipase from Pseudomonas Sp.RT-1,TQ925
  6. Breeding of Fungal α-amylase High-producing Strain by Genome Shuffling,TQ925
  7. Research on Anaerobic Fermentation Processing Technology of Dead Tilapia,TS254.4
  8. Characterization and Identification of Maturity of Pig Manure Composting,S141.4
  9. Effects of Fermentive Cornstalk Culture Medium Mixture Ratio on Physical and Chemical Characters in Potted Peony,S685.11
  10. Effects of 2-Hydroxy-4-Methionine Isopropyl on Rumen Fermentation and Production Performance of Holstein Cows,S823.8
  11. Study on the Breeding of High Efficient Detoxification Strains and Suggestion Parameters of Solid Fermentation on Cottonseed Meal,S816.6
  12. Study on the Secondary Metabolities from Two Fungi,S482
  13. The Effectr of Lactic Acid Bacteria and Rice Bran on Dynamic Change of Rice Straw Silage Fermentation,S816.53
  14. Sophorose the extraction and purification for the inter- type Neurospora cellulase produced induction of,TQ925
  15. Expression of Glucose Oxidase Gene from Aspergillus Niger Z-25 in Pichia Pastoris,Q78
  16. The Influence of Antibiotics on Yogurt During the Fermentation,TS252.54
  17. Studies on Optimization Technology of Fermentation with Corn Straw and Cow Dung Mixed Material,S216.4
  18. Insecticidal Activities of Recombinant CryIAc Protein from Bacillus Thuringiensis and Solid Fermentation Products from Aspergillus Terreus PNR2,S476.1
  19. Studies on the Appliction of Organic Nitrogen Fertilizers to Soilless Culture in Cucumber,S642.2
  20. The Study of Adding Wet Brewer’s Grains on Fermentation Quality of Mixed Ensilages of Corn Stalk and Agricultural Byproducts,S816.53
  21. Study on Nutritional Evaluation of Feed for Ruminants by in Vitro,S816

CLC: > Industrial Technology > Chemical Industry > Other chemical industries > Fermentation industry > General issues > Fermentation process
© 2012 www.DissertationTopic.Net  Mobile