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Preparation, Characterization and Analytical Applications of Rare Earth Fluorescent Probes

Author: SunSiLing
Tutor: XuShuKun
School: Northeastern University
Course: Analytical Chemistry
Keywords: Terbium complexes DNA LaF3: Eu3 Fe3
CLC: O641.4
Type: Master's thesis
Year: 2008
Downloads: 144
Quote: 1
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Abstract


Due to the unique structure of the electron shell of the rare-earth ions, the emission wavelength of the rare earth fluorescent probes from the impact of the external environment, and narrow emission spectra and photochemical stability, fluorescent advantages of long life, so get extensive attention in the field of analysis has broad application prospects. In this study, 1,10 - phenanthroline, γ-methacryloxypropyl trimethoxy silane (KH-570) as ligand was prepared in anhydrous ethanol solution terbium binary and ternary complexes. The structure of the complexes were characterized by UV spectra and infrared spectroscopy, and fluorescence spectroscopy to study the luminescent properties of the complexes. Connected with the Y-aminopropyl triethoxysilane ternary complexes of to bring amino, and this amino complex as a fluorescent probe, using time-resolved fluorescence method herring sperm DNA was measured. The results show that the fluorescence intensity of the complexes is enhanced with the increase of the concentration of the herring sperm DNA, and both linear relationship exists within a certain range, thereby achieving a quantitative detection of herring sperm DNA. The linear range of 0.300-4.70 mg / L, and the detection limit of 0.08 mg / L concentration of 3.00 mg / L of herring sperm DNA parallel determination of 11 times, and the relative standard deviation of 0.8%. Lanthanide complexes in aqueous solution with a water ligand, resulting in the emission intensity decreases, thus limiting its application in the analysis. And rare earth nanomaterials make up for the deficiencies of the complexes in these areas to provide new ideas and ways for further research and application of rare earth fluorescent probes. This study were used the organic phase method, and the aqueous phase synthesis LaF3: Eu3 nanoparticles, the fluorescence properties characterized by fluorescence spectroscopy. Water phase method synthesis of rare earth nanoparticles application XRD, TEM techniques to characterize the structure. , As compared with the organic phase to aqueous phase synthesis of LaF3: of Eu3 surface of the nano-particles with a hydrophilic group, the fluorescence intensity is high, and therefore can be applied to analysis field. The first time the aqueous phase to prepare nanoparticles for Fe3 Determination results show that the the Fe3 presence of fluorescence intensity of rare earth doped nanoparticles system weakened, and Fe3 concentration of 5.00 × 10-6-5.00 × 10-4 mol / L range and rare earth nano-particle fluorescence intensity quenching showed a good linear relationship between the method detection limit of 1.25 × 10-6 mol / L. Concentration of 5.00 × 10-5 mol / L of Fe3 parallel determination of 11 times, and the relative standard deviation of 0.5%. The spectrophotometry results of the method can be used for the determination of the iron content of beer, and measuring the results with the classical.

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CLC: > Mathematical sciences and chemical > Chemistry > Physical Chemistry ( theoretical chemistry ),chemical physics > Structural Chemistry > Complex chemistry ( coordination chemistry )
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