Dissertation > Excellent graduate degree dissertation topics show

Construction of Labelling Vaccines of Brucella and the Establishment of Differential Diagnosis

Author: DuanXiaoYu
Tutor: WangXingLong
School: Jilin University
Course: Preventive Veterinary Medicine
Keywords: Molecular marker vaccine strains P39 Suicide plasmid Homologous recombination
CLC: S854.43
Type: Master's thesis
Year: 2007
Downloads: 180
Quote: 1
Read: Download Dissertation


Brucella P39 cytoplasmic binding protein is a good T- cell antigen can induce strong delayed -type hypersensitivity , and produce large amounts of INF -γ , so that it is recognized as a DNA vaccine candidate molecules . The purified recombinant P39 for serological diagnosis of animal brucellosis is an excellent antigen . P39 protein after transformation strains inoculated animal , the animal will not be able to P39 immune response , the P39 protein can be used as molecular markers to determine whether the animal is a natural strains . Brucella Rev.1 gene encoding P39 protein was amplified by PCR , sequencing and cloned into the cloning vector pB ] LlescipLSKII ( ) to construct the recombinant vector pBp39 then a section of pBp39 encoding P39 1008bp fragment replaced kan resistant fragment constructed suicide the recombinant plasmid pBp39 -kan . Electricity conversion method pBp39-kan Ru Bulu coli for suicide plasmid due pBp39-kan , pre-designed Kan resistance fragment is homologous recombination sequences rescued in the side of each of the P39 gene arm homologous recombination in the form of a double exchange , so that led to the replacement of the P39 gene , thereby building became who rouxii bacteria molecular marker vaccine strains . Antigen P39 protein expression were detected infected with Brucella bacteria and new build molecular marker vaccine strain of animals , such as test results are negative , then the the animal artificial immune ; positive test results , it shows that the animals as a natural infection .

Related Dissertations

  1. Construction and Characterization of a Salmonella Choleraesuis C78-1 △crp Deletion Mutant,S852.61
  2. Construction of Recombinant Adenovirus Vector Co-expressing P12A and 3C Genes of FMDV O Virus,S852.65
  3. Construction Expression Cellulase Recombinant Strain of Lactobacillus Reuteri Without Resistance Marker,S816
  4. Construction and Expression of Marker-free Targeting Vector of Human Leukemia Inhibitory Factor,R733.7
  5. Relationship between the Mutation of KatG in Mycobacterium Tuberculosis and Drug-resistance of INH,R52
  6. Clonging and Functional Identification of the Genes luxS and pilA Related To Biofilm Formation of Haemophilus Parasuis,S852.61
  7. Construction of Recombinant Adenovirus of VP2 Protein of Infectious Bursal Disease Virus JS Strain and Biological Characterization of the Recombinant Virus,S852.65
  8. Study on Irp1 and Irp2 Genes of HPI in Escherichia Coil,S852.61
  9. Construction of SEF14 Fimbrial Deletion Mutants from Salmonella Enteritidis and Analysis of the Related Functions,S852.61
  10. Expression and Activity of Cdk5/p39 during Rats Vc Development,R745.11
  11. Construction and Characterization of Attenuated Salmonella Typhimurium ΔcrpsL1344 and Δcyasl1344,S852.61
  12. Potato late blight resistance gene molecular transformation and Xanthomonas oryzae IAA biosynthesis genes identified,S435.111.4
  13. Rabbit hemorrhagic fever virus recombinant vaccine Construction and screening,S858.291
  14. Application of enhanced green fluorescent protein of Streptococcus suis type 2 and real-time quantitative PCR analysis in vivo infection dynamics,S858.28
  15. A High Efficient Gene Targeting in Multiple Locus Induced by Zinc Finger Nucleases,Q78
  16. Construction of a Recombinant Marek’s Disease Virus Expressing the Fusion Protein of Newcastle Disease Virus Strain ZJ1,S852.65
  17. RAD51 in bladder cancer tissues,R737.14
  18. Construction of Recombinant Adenovirus Vector Co-expressing the E and M Gene of Porcine Reproductive and Respiratory Syndrome Virus,S852.65
  19. With rabbit myxoma virus as vector rabbit hemorrhagic fever virus recombinant vaccine research,S858.291
  20. Construction and Characterization of a ApxⅣ △orf1 Mutant of Actinobacillus Pleuropneumoniae,S852.5
  21. Complete Genome Sequencing of Actinobacillus Pleuropneumoniae Strain JL03 and Structural Analysis of the flp Operon,S852.61

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Veterinary clinical medicine > Veterinary diagnostics > Laboratory diagnostic method
© 2012 www.DissertationTopic.Net  Mobile