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30 1 -month-old Lanzhou fat-tailed sheep, lambs , were randomly divided into six groups . 1,3,5,7,9,11 and 13 -month-old to slaughter , collected omental adipose tissue , liver tissue , muscle tissue , subcutaneous adipose tissue , perirenal adipose tissue and tail adipose tissue , and the use of improved TRIzol extraction of total RNA , the SuperScriptTM III First -Strand Synthesis System for RT - PCR kit reverse transcription of the organizations , the various stages of cDNA . Design specific PCR primers , selected 18S rRNA GADPH PGK1 , SDHA TUBB YWHAZ and ACTB seven housekeeper gene Lanzhou big tail sheep 7 growth and development stage , six kinds of organizations in the temporal and spatial expression conduct real-time quantitative PCR ( qPCR ) were analyzed using geNorm , NormFinder , and BestKeeper three software to analyze the stability of gene expression , screened expression the most stable GADPH TUBB2 or YWHAZ and TUBB2 gene research Lanzhou fat-tailed sheep fat metabolism gene space-time qPCR the dual- gene expression internal reference factor . Study after screening the stable expression the GADPH and TUBB2 of genes stably expressing the geometric mean of the coefficient of the expression of FAS, DGAT1, HSL, H-FABP, UCP3, G6PD and ACCα7 a target functional genes qPCR homogenization show that , Lanzhou fat-tailed sheep fat metabolism gene FAS, DGAT1, HSL, FABP and G6PD expression pattern overall in Shen lipid organization was \larger. The ACCα, HSL and G6PD gene expression in Lanzhou fat-tailed sheep Shen lipid organization mode in different organizations exist significant differences, in particular, unlike other adipose tissue changes in adipose tissue in the tail . UCP3 gene expression in the liver and muscle tissue , expression in adipose tissue is very low , almost no expression . Description : FAS , of DGAT1 , HSL , FABP and G6PD gene Lanzhou fat-tailed sheep tail fat - specific traits --- excessive deposition , candidate genes for further study can be used as partial deposition of Lanzhou fat-tailed sheep fat . ACCα, HSL and G6PD gene can be used as further study of candidate genes localized deposition of Lanzhou fat-tailed sheep fat . The UCP3 gene Lanzhou fat-tailed sheep fat local deposition . This study provide a theoretical basis for further exploration of Lanzhou fat-tailed sheep fat metabolism and fat deposition law .
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