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Mycorrhizal Fungi Resistance Mechanism to Poplar Canker Disease

Author: ZhanWei
Tutor: TangMing
School: Northwest University of Science and Technology
Course: Forest Protection
Keywords: Mycorrhizal fungi Poplar Canker Resistance
CLC: S763.7
Type: Master's thesis
Year: 2010
Downloads: 82
Quote: 0
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Abstract


The papers by the the poplar fungal disease pathogens separation, identification, will be isolated from the Poplar Canker Pathogen the poly students Koana globosum (Dothiorella garigaria Sacc) mycorrhizal fungi on poplar mixed inoculation study poplar growth and resistance substance content changes, explore the ability of mycorrhizal fungi to improve Poplar anti-ulcer disease. Draw the following conclusions: 1. The poplar fungal disease pathogens separation, identification collected from 10 kinds of poplar 35 diseases specimens were isolated the poplar pathogenic fungi 14 ascomycete subphylum 3 12 4 species half know bacteria subphylum 2 2 2 species. 14 fungal ITS-rDNA sequencing and phylogenetic tree. Bacteria in GenBank of the two the polyethylene students Koana shell has been submitted sequence similarity coefficient and separation factor are compared to determine the research poplar ulcer pathogens the poly students Koana globosum a666 a669b two. 2 The role of mycorrhizal fungi on poplar growth and anti-ulcer disease arbuscular mycorrhizal fungi (Glomus mosseae, GM) and ectomycorrhizal fungi (Boletus luridus, BL) inoculated poplar (Populus x euramerieana.cv. \74/76 \Inoculated with arbuscular mycorrhizal fungi and ectomycorrhizal fungi poplar chlorophyll, soluble protein content and the strength of the root activity increased by 80% and 57% compared with the control, respectively, 47% and 51%, 32% and 49%, when arbuscular mycorrhizal and ectomycorrhizal pathogen invasion, forming poplar incidence and disease index decreased by 58.7%, 57.6% and 40%, 53.7%. 3. Mycorrhizal fungi Poplar Poplar Resistance Associated Enzyme and other substances to form mycorrhizal infection by pathogens could increase resistance-associated enzyme activity, formation of arbuscular mycorrhizal and ectomycorrhizal poplar superoxide dismutase, phenylalanine knot the ammonia enzyme activity in the highest value when compared with the control were increased by 80%, 43% and 75%, 47%. AMF treatment effectively promote the of susceptible poplar body free proline accumulated, reducing the content of malondialdehyde (MDA), compared with the control have reached a very significant difference (P lt; 0.01), the formation of Cong mycorrhizal and ectomycorrhizal poplar proline, MDA activity at the highest value, respectively, compared with the control to raise and lower the 73%, 54% and 63%, 27%. Arbuscular mycorrhizal fungi is better than inoculated ectomycorrhizal fungi. 4. Mycorrhizal fungi induced a sense of disease poplar pathogen-associated protein expression in different types of mycorrhizae formed before and after infection poplar susceptible parts of an increase in total protein content compared with the control, and the improvement of the total protein content and poplar antiviral The positive correlation, that after induction of pathogen-associated protein. The formation of the ectomycorrhizal the poplar by pathogen infection chitinase, β-1, 3 - glucanase and pathogen-associated protein is suppressed; arbuscular mycorrhizal poplar seedlings by pathogen infection, several small chitinase, β-1, 3 - glucanase and pathogen-associated protein can be induced to express. Arbuscular mycorrhizal fungi in poplar seedling period plays an important role in improving poplar antiulcer disease.

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CLC: > Agricultural Sciences > Forestry > Forest Protection > Forest Pest and Disease Control > All kinds of tree pests and diseases and their prevention
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