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The Physiological and Biochemical Mechanism of the Response of Tung Oil Tree’s Flower Bud Differentiation to Hormone Signal

Author: ZuoAiLing
Tutor: LiJianAn
School: Central South University of Forestry Science and Technology
Course: Forest cultivation
Keywords: Tung tree Endogenous hormones Nutrient elements Flower bud differentiation
CLC: S794.3
Type: Master's thesis
Year: 2010
Downloads: 74
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Abstract


The tung tree has a unique biological characteristics, and potential of the forest model plant. Used in this experiment has a unique early flowering characteristics years Tong as the research object, through its different levels of exogenous 6-BA, ABA, GA treatment of exogenous hormone tung tree endogenous hormones, nutrients and Flower Bud Differentiation in order to understand the physiological and biochemical mechanism of the tung flower bud differentiation hormone signal response, reveal the internal relations of hormones and nutrition during flower bud differentiation and interaction, provide the basis for further regulation of flowering, flowers and fruits management. In this thesis, the results are as follows: 1, spraying 6-BA, ABA can effectively improve the tung tree bud. One of the most obvious role in promoting treatment B2 (ABA 100mg / L) and A1 (6-BA 25 mg / L), flower bud differentiation rate of 88.86% and 86.83%, 41.28% and 39.26%, higher than the control; while processing the A3 ( 6-BA 100mg / L), B3 (ABA 200mg / L) is not obvious effect on flower bud differentiation; spraying GA, reduce the rate of flower bud differentiation is C3 (GA 200 mg / L), inhibition of the more obvious. By analysis of variance showed that, in addition to A3, B3, with the control difference was extremely significant. 2 in flower bud physiological differentiation and morphological differentiation of pre-, 6-BA, ABA treatment of total soluble sugar and starch content of an upward trend. Show a downward trend in the late bud morphological differentiation, total soluble sugar content. GA treatment of total soluble sugar and starch content trends similar to the first two exogenous hormones, but its content is basically the same or lower than the control with the control. Description of exogenous hormones, transport and distribution capabilities of the total sugar impact, to adjust the variation of the C / N, thereby impact into flower. 3, soluble protein first increased and then downward trend in the flower bud differentiation, show that differentiation requires the accumulation of large amounts of protein. GA treatment significantly reduced the protein content, and 6-BA and ABA treatment role in promoting the improvement of the soluble protein. 4, in the flower bud physiological differentiation period, 6-BA and ABA treatment can significantly reduce the content of plants of IAA and GA, to improve the content of ABA and ZR. GA on the contrary, can significantly improve the content of the plants of IAA and GA, to reduce ABA and ZR content. Overall, in the flower bud differentiation stage, the endogenous GA content changes slightly, the lowest content as measured by the four hormones. In flower bud physiological differentiation period, IAA, GA, ABA and ZR content has a peak rise late, IAA, GA decreased volatility, ABA and ZR continued to decline in the bud morphological differentiation. In flower bud physiological differentiation period, IAA, Ga, ABA and ZR content increased at the same time, help to maintain the balance of ABA / IAA, ZR / GA, to help promote flower bud morphological differentiation, improve the quality of flower buds. 5,6-BA and ABA treatment on the ZR / GA the Zr / IAA, ABA / IAA and ABA / GA ratio improve obviously promote. Wherein the processing A1, A2 (6-BA 50 mg / L), B2, B1 (the ABA 50mg / L) of the effect is greater than the ZR increasing the content of the key factors is to promote flower formation rate to increase and improve the quality of flower buds.

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CLC: > Agricultural Sciences > Forestry > Forest tree species > Special use of broad-leaved class > Tung
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