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The sea buckthorn is extremely broad distribution of a native tree species in Northern China, and has a significant ecological benefits, social and economic benefits. With the deepening of the sea buckthorn research, development and utilization of seabuckthorn resources has attracted more and more attention in the community. China Seabuckthorn mainly seabuckthorn ecological benefits, biochemical composition and economic use value for seabuckthorn under Stress less adaptive research, especially about seabuckthorn different varieties in drought stress conditions variation of different physiological and biochemical indicators to be further in-depth study. Based on the above situation, the paper selects Zhangye City, Gansu Province, is more widely planted seabuckthorn, Liao Fu One, Liao Fu II, muddy gold, orange, Qi spine six kinds (varieties) of sea buckthorn, a variety of (species) seabuckthorn photosynthetic rate, transpiration rate, leaf water content, the critical saturation deficit, chlorophyll content, MDA content, free proline content, SOD activity, POD activity, membrane permeability, physiological and biochemical indicators of change under drought stress The case was measured. At the same time the use of subordinate function values ??analysis and principal component analysis method to evaluate the drought resistance of a variety (varieties), the use of cluster analysis classified the six kinds (species) of sea buckthorn. Through the above study, the following conclusions: (1) six kinds of sea buckthorn (varieties) of photosynthetic rate and transpiration rate has a significant diurnal variation. Stress early in June, and a variety (varieties) Diurnal Variation showed a bimodal curve, light lunch break \The left and right down to the minimum; In addition to orange, the remaining five species (varieties) seabuckthorn process of transpiration rate curve is approximated by a single peak, peak around 11:00. After drought stress, in September, the various species of photosynthetic rate and transpiration rate decreased; Liao Fu One, Qi spine, muddy gold, four kinds of Chinese cultivars (varieties) of sea buckthorn photosynthetic rate curves for the two peaks, Liao Fu two number two orange varieties photosynthetic rate curve for a single peak, and its peak at around 11:00; various (varieties) of the process of transpiration rate curve bimodal performance, and maximum values ??in 11: minimum around 13:00 or so, 00 and 15:00. Various (varieties), One and Liao Liao Fu Fu II photosynthetic rate and transpiration rate highest muddy gold photosynthetic rate and transpiration rate the lowest in six species (varieties). (2) With the intensification of drought stress, the leaves of a variety (varieties) moisture content decreased, the critical saturation deficit values ??rise. More stable in the entire process of drought stress, leaf water content of a variety (varieties), between 46.68% to 69.34%, Qi spine, muddy gold, orange three kinds (species) of the lower water content, Liaoning Fu II leaf water content for a variety of the highest (varieties). Seabuckthorn critical saturation deficit values, Qi spine in the late critical saturation deficit (varieties) minimum for a variety of its vulnerability to drought stress effects. ③ six kinds (species) Leaf chlorophyll a, chlorophyll b content with the intensification of the drought stress decreased carotenoid content showed a trend first and then decreased. Liao the Fau One of three pigment content in the highest of the six kinds (species), three orange pigment content is lowest. ④ various (varieties) as drought stress intensifies, free proline content showed an increasing trend for the sustained, Liao Fu first, Liao Fu II, China the seabuckthorn the free proline content. ⑤ With the increase of drought stress, SOD activity and POD activity similar trend, showing a trend first and then decreased. Stress process, Liao Fu-1, Liao Fu II, three kinds of Chinese cultivars (varieties) SOD activity and POD activity were higher, Qi spine POD activity and SOD activity has been the weakest. Chinese the seabuckthorn three varieties of SOD activity decreased in the late Liao Fu One, Liao Fu II, compared with other species (varieties) slow, they maintain the ability of SOD activity, better drought resistance. ⑥ in the stress process, various (varieties) Leaf MDA content have different levels of accumulation, and with the stress, the increase in MDA content of a variety (varieties) seabuckthorn larger, Description Seabuckthorn film system hurt gradually enhanced. Orange, muddy gold, Qi spine three varieties of MDA content has been at a high level, and Liao Fu No.1 and Liao Fu II, MDA content were lower throughout the stress period, indicating that these two varieties membrane system damage light drought resistance. (7) With the intensification of the degree of drought stress, a variety of sea buckthorn (varieties) cell membrane permeability showed a trend of continuous increase cell membrane permeability entire stress process from small to large: Liao Fu II, Liao Fu One Chinese seabuckthorn Qi spine, muddy gold, orange, Liao Fu II Drought best orange under drought stress by the degree of injury heaviest, its worst drought resistance. The ⑧ this study various seabuckthorn (varieties) in drought stress measured most indicators showed values ??similar to various indicators of stress early, but with the trend of increased stress, exacerbated by luffing and differences. Mild water deficit, sea buckthorn has some adaptability, and thus able to maintain a small change in each indicator, when the stress level exceeds seabuckthorn the limits of self-regulation, drought stress of seabuckthorn injury aggravated, so indicators luffing With stress was intensified and increased. ⑨ through the use of principal component analysis and subordinate function values ??of the six kinds of sea buckthorn (varieties) drought resistance evaluation, the evaluation results of the two methods, drawn six kinds (species) seabuckthorn drought resistance in order from strong to weak order : Liao Fu II gt; 1 Liao Fu gt; Chinese seabuckthorn gt; orange gt; muddy gold gt; Sarkozy spine. By cluster analysis, six kinds of sea buckthorn (varieties) is divided into the following two categories: the first category 1 Liao Fu Liao Fu II, three kinds of Chinese cultivars (varieties), the second category is the orange, muddy Jin, Qi spine three kinds (species). By hierarchical clustering pedigree chart shows, Liao Fu No.1 and Liao Fu II drought resistance of six kinds (species) most similar nature, and the Chinese seabuckthorn drought slightly worse than the Liao Fu varieties, but superior The other three varieties. At the same time, through the selection of drought resistance evaluation, drought resistance evaluation method selection, Seabuckthorn Variety Breeding three elements discussed following recommendations were made: drought resistance evaluation index system should be a multi-index, multi-angle, wide The scope of the principle of the selection of indicators; Drought evaluation method, should strengthen the correlation coefficient between the regard to various indicators, screened a representative and a significant indicator of the importance of, and then proceed to the evaluation to ensure that the evaluation method is reasonable, reliable evaluation results . Seabuckthorn Variety Breeding considering the seabuckthorn various benefits, fruit quality, adversity adaptability, in order to achieve a stable, high-yielding purpose of better play a Seabuckthorn effective. Finally, the prospects of the use of resources of the sea buckthorn prospect.
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