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Nano-ZnO preparation, characterization, and its modified electrode electrocatalytic biomolecules

Author: HongMei
Tutor: XiaoYaPing；DaiZhiZuo
School: Nanjing Normal University
Course: Physical and chemical
Keywords: Zinc oxide Horseradish peroxidase Glucose oxidase Dimethyl sulfoxide Biosensor
CLC: O614.241
Type: Master's thesis
Year: 2007
Downloads: 311
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Abstract


In the study of the biological sensor and the fuel cell, etc., the fixing of biological macromolecules is a very important area of ??research. In the fixed aspects of the biological macromolecules, and the choice of vector is very important. Taking into account the nano ZnO is an inorganic semiconductor materials, and its preparation method is simple, inexpensive, and has a larger specific surface area, etc. In this thesis, the preparation of nano-ZnO, characterization, and nano-ZnO carrier horseradish peroxidase objects enzyme (HRP) and glucose oxidase (GOx) modified electrodes were prepared and their electrochemical and electrocatalytic performance. The main results are as follows: 1. Direct precipitation method got ZnO nanoparticles. And X-ray diffraction (XRD), transmission electron microscopy (TEM), X-ray energy dispersive spectroscopy (EDS) a variety of techniques such as morphology and structure of ZnO nanoparticles were characterized. Found that the synthesis of ZnO by direct precipitation method showed Quartet cone, with a porous structure, uniform size, good dispersion, there is no obvious agglomeration. The average particle diameter of about 65nm, BET value 43m 2 / g, a pore size of 4nm. 2. Fixed in a mixture of ZnO and dimethyl sulfoxide (DMSO) HRP modified electrode surface electrochemical and electrocatalytic properties. The results show that, not denatured HRP modified electrode surface is fixed in the ZnO-DMSO, direct quasi-reversible electrochemical reaction, the appropriate amount of potential to -228 mV. Amount of potential and the pH of the solution was a linear relationship between the direct electrochemical reaction of HRP 1 electron 1 proton surface control process. Electron transfer rate constant k s 3.95 ± 0.89 s -1 , than those reported in the literature value, indicating that due to the synergistic effect of DMSO and ZnO, so fixed in the ZnO -DMSO-modified electrode surface, HRP fast electron transfer can be performed. It also found that fixed in the ZnO-DMSO modified the electrode surface HRP H 2 O 2 restore good electrocatalytic activity, stability and reproducibility. 3. Fixed in ZnO modified electrode surface GOx electrochemical and electrocatalytic properties. The results show that, not fixed in ZnO modified electrode surface GOx degeneration, quasi-reversible electrochemical reaction directly, the appropriate amount of potential to -456 mV. The linear relationship of the amount of potential and the pH of the solution described GOx direct electrochemical reaction is a process of a 2-electron-proton. Electron transfer rate constant k s 39.2 ± 7.2 s -1 , have a good value is much larger than that reported in the literature show that the ZnO GOx direct electrochemical reaction facilitating role. Also been found that after adding glucose and oxygen consumption, the oxygen reduction peak decreased, and with added glucose, oxygen reduction peak linear decline, so the method may build a new type of glucose sensor and a higher sensitivity. This the Gox-znO electrode can also be used for bio-fuel cell and therefore has good application prospect.

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CLC: > Mathematical sciences and chemical > Chemistry > Inorganic Chemistry > Metal elements and their compounds > Section II group metal element and its compounds > The zinc Vice family (Ⅱ B group metal elements ) > Zinc Zn
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