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In this study, has anti-cancer, anti-oxidation, and other important physiological activity and turmeric have broad market prospects for the study, the use of supercritical fluid extraction, microwave extraction, molecular distillation, natural clarifying agent flocculation, macroporous resin, and other new centrifugal chromatography extraction and separation technology, turmeric turmeric, curcumin extraction, separation and purification process was thorough and systematic study and analysis of better screening methods, processes and purification conditions; product obtained by means of UV, HPLC , GC-MS, LC-MS instruments and other equipment for qualitative and quantitative analysis, the development of new drugs that can be used in three series of high-purity active compounds and functional activity of turmeric products, for the first time developed a highly efficient environmentally friendly green new integrated optimization technology, has made a series of innovative results: 1. curcumin ingredient four kinds extraction technology system is relatively findings. Supercritical fluid extraction, solvent reflux extraction, ultrasonic extraction and microwave extraction were compared. The results showed that: solvent reflux extraction, ultrasonic extraction and microwave extraction method than the supercritical fluid extraction method is more suitable for the extraction of curcumin. Experimental results three kinds of extraction methods optimized curcumin yields were 2.53 percent, 3.22 percent and 3.61 percent. Curcumin extraction, microwave extraction method is more efficient, time-saving advantages. 2 ginger and turmeric butter Supercritical - microwave extraction technology of combined results. C02 supercritical fluid combination of static and dynamic gradient boost and ginger butter extraction technology research, experiment optimization, ginger butter yield reached 8.95%, compared with steam distillation increased by 4 times. Supercritical C02 extraction of Turmeric Turmeric powder as raw material after the system of microwave extraction process curcumin, the experiment optimization, curcumin yield reached 3.22 percent. Because ginger butter from extracted by supercritical advance so that after curcumin extract easy to refining, to some extent, reached a ginger butter and turmeric pigment extraction, separation while achieving results. 3 turmeric extract supercritical CO2 extract and steam distillation GC / MS identification of component analysis results of the comparison. Component of the total ion current show: supercritical method can more effectively keep the raw ingredient of ginger butter, distilled by steam distillation of low boiling point component material losses. Supercritical steam distillation method with the resultant compositions species is insignificant, but some components of the relative percentages are quite different, in which the highest content of seven kinds of ingredients are two ways to extract the relative content are: α-turmeric ene (8.53%, 3.92%), α-zingiberene (5.61%, 20.25%), β-myrrh ene (1.72%, 2.01%), β-sesquiphellandrene (8.83%, 10.83%), aryl Turmerone (31.21%, 10.28%), α-Turmerone (17.57%, 27.08%), β-Turmerone (11.74%, 8.38%). 4 Turmeric supercritical extract a molecular distillation purification of the results. Molecular distillation separation and purification of the supercritical extract of turmeric, draw a purification process as follows: operating pressure 10Pa, distillation temperature 80 ℃, condensing temperature 2 ℃, feed rate 3mL/min, feed temperature 45 ℃, the membrane wiper speed 300r / min, steamed content reached 81.05%, effectively preserved ginger butter active ingredient. 5 molecular distillation purified by distillation separation and enrichment of the two studies. Purified on a molecular distillation separation and enrichment for two, purified by high resolution gas chromatography - mass spectrometry identification, ginger butter derived oxygenated sesquiterpenes and sesquiterpene ratio of 1.23:1, respectively, from the raw material change for the boil-component distillation residue of 0.35:1 and 1.84:1 component in, help oxygenated sesquiterpene sesquiterpene different functions and development of deep processing products. 6 curcumin extract purification findings. Turmeric supercritical - microwave extracts using natural clarifying agent flocculation, macroporous resin purification curcumin combined technology enrichment get purified. Under the optimized process conditions, to a purity of 85.3% of curcumin, this process is less solvent consumption, easy industrialization amplification. 7 curcumin compounds LC-MS analysis results. Application of liquid chromatography - electrospray ionization mass spectrometry (LC-MS) coupling technique to determine the class of the three compounds curcumin molecule in electrospray ionization mass spectrometry, positive and negative ion detection mode cracking characteristics, helps to determine the molecular weight of these compounds and structural identification.
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