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Blue eye daisy (Osteospermum spp.) Is very good and potted flowers, dense flowers , brightly colored , strains clump plump , both color and shape, the beauty of the rhyme ornamental value worth promoting planting . The blue-eyed daisy in our garden fresh application , seeds are mostly introduced from abroad , the cost is very high . Therefore, the new germplasm breeding and improvement work is extremely important . The papers focus on blue-eyed daisy fine individual screening and breeding technology to provide a certain theoretical and technical foundation for the the blue eye daisy own breeding and rapid promotion . The main findings are as follows : ( 1) fine individual choice based on the Field Biology traits observational records , ornamental total evaluation criteria established seven evaluation ( color, flower , flowering days , the amount of flowers , flower diameter , plant type , plant height ) , Analytic Hierarchy Process to establish a set of comprehensive evaluation model suitable for blue-eyed daisy , a comprehensive evaluation in accordance with the comprehensive evaluation model , the 140 blue - eye daisy monoclonal seed propagation , selected 12 with development potential fine individual . '109 ' , '093 ' , '125 ' , '068 ' , '129 ' And '011' suitable ground cover plants , '089 ', '138 ', '102 ', '118 ', '067 ' '045 ' suitable for potted plants . ( 2) stem segments Cluster shoots induction of stem with axillary bud explants induce adventitious buds on MS 6-BA0.2mg / L , 12 individuals clones . (3) Establishment of regeneration system leaves no vaccine material , the blue eye daisy regeneration system . The experimental callus induction medium MS 6-BA1.0mg / L NAA0.5 mg / L; induce callus differentiation buds best medium MS 6-BA1.0 mg / L the NAA0.1 mg / L or MS , 6 - BA1.0 mg / L NAA0.5 mg / L ; rooting medium 1/2MS NAA0.5mg / L effect . (4 ) cutting propagation of low concentrations of NAA treatment can improve the rooting of cuttings , high concentrations of NAA (> 150 mg / L) inhibition Rooting of 150 mg / L NAA treatment Rooting best results .
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