Dissertation > Excellent graduate degree dissertation topics show

Purification and Characterization of Pepsinnogens Pepsins from Sea Bream and Mandarin Fish

Author: ZhouQiong
Tutor: SuWenJin；CaoMinJie
School: Jimei University
Course: Of Food Science
Keywords: Yellow fin bream Mandarin fish Pepsinogen Pepsin Purification Properties of
CLC: S917.4
Type: Master's thesis
Year: 2007
Downloads: 216
Quote: 0
Read: Download Dissertation

Abstract


Characteristics of fish pepsin is an important one in the fish physiology and biochemical studies , but also fish with the feed - developed theoretical foundation based on farmed fish bait configuration has important guiding significance . The seawater yellow fin snapper and freshwater mandarin fish pepsin original purification and enzyme nature of research , so far , have not been reported at home and abroad . Materials seawater yellow fin snapper and freshwater mandarin fish , its stomach purified by ammonium sulfate precipitation , DEAE-Sephacel ion exchange and SephacrylS-200 gel filtration method for the first time four pepsinogen and enzyme properties , to provide experimental evidence for the development and utilization of pepsin , and also lay the foundation for the two great economic value fish digestive physiology research . SDS-PAGE showed purified four kinds of yellow fin bream pepsinogen (PG-I, PG-II, PG-III and PG- IV) of the molecular weight of 36,32,32 as well 34kDa ; mandarin fish four stomach pepsinogen ( PG -I and PG - II , PG - III ( A ) and PG-III (b)) of the molecular weight of approximately 36,35,38 well 34kDa . PH2.0 under each pepsin original can be converted in a short time to become active state pepsin , their relative molecular weight of approximately 30kDa . Acid - denatured bovine hemoglobin as substrate , four yellow fin snapper pepsin optimum pH of 3.0-3.5 , and the optimum temperature of 45-50 ° C . Four mandarin fish pepsin optimum pH of 3.5 , and the optimum temperature of 40-45 ° C . The eight kinds of pepsin are acidic proteases ( aspartic protease ) , Pepstatin they are effective inhibitors of slightly different , but the inhibitory effect . Four yellow fin snapper pepsin acid - denatured hemoglobin Km values ??were : 8.7 × 10-8,1.0 10-7,8.6 × 10-8 and 7.3 x 10- 8 mol / L , mandarin fish Km values ??were : 2.3 × 10-7,1.2 × 10-7,2.4 × 10-8 and 1.3 x 10-7mol / L . Yellow fin snapper PG-I and PG-II system was the primary antibodies for Westernblot display PG-II can occur strong immunological cross-reactivity with the yellow fin bream four protease , and PG-I react strongly with PI weak reaction , and P-II does not react with the other two proteins . Analysis of the the mandarin fish PG-I and PG-II N-terminal 12 amino acid sequence , and found that 92 % homology between the two .

Related Dissertations

  1. A Research on Factors of the Establishment American Family Properties Decalaration System,D771.2
  2. The Preparation and Thermoelectric Properties of Rare Earth Subsituted Ag Composite Ca3Co4O9-Based Oxides,TQ174.1
  3. Synthesis, Structure and Properties of PBO/SWNT Composite Fibres,TQ340.64
  4. Preparation and Tribological Properties of TZ3Y20A-SrSO4 Ceramic Matrix Composites,TB332
  5. Reactive Hot-Pressing and Mechanical Properties of （ZrB2-ZrO2）/BN Composites,TB332
  6. Research on Welding Characteristics of CO2 Laser-GMA Hybrid Welding for Ultra-Low Carbon Bainitic Steel,TG456.7
  7. Microstructure and Mechanical Properties of Mg-Zn-Y-Zr Alloys,TG146.22
  8. Study on Protective Effects of Apple Polyphenol on Immune System Injury Caused by γ-Ray,S661.1
  9. Simulation and Experimental Study on the Scattering Properties of Two Dimensional Rough Surfaces,TP391.41
  10. Study on Wastewater Purification Effect of the Free Surface Wetland with Different Substrates and Plant Combination,X703
  11. Penicillium Expansum TS414 Lipase in Pichia Pastori: Expression, Purification and Enantioselective Esterification of (R, S)-naproxen,Q814
  12. Urothermal Synthesis, Structure and Characterization of Functional Coordination Polymers,O631.3
  13. Studies on Fermentation Optimization, Purification and Enzyme Characteristics of Lipase from Aspergillus Oryzae FS-1,TQ925.6
  14. Studies on Isolation, Purification and Chemical Structure for Polysaccharides from Camellia Chrysantha(Hu) Tuyama,S567.19
  15. Research of Glycosaminoglycan from Perna Virids Effect on Blood Lipid,R285.5
  16. Effects of the Exotic Invasive Plant Solidago Canadensis L. on the Community Structure of Soil Anmals,S451
  17. Purification of Endogenous Proteases from (Litopenaeus Vannamei) Shrimp Head and Their Enzymology Properties,S985.21
  18. Rock-fill Concrete Experiments on Comprehensive Properties and Studies on Thermal Stress,TU528
  19. Effects of Enzyme/Chemical Agents on Refining of Plant Fiber,TS713
  20. Study on the Preparation of the Low Molecular Weight Fuciodan from Sargassum Henslowianum (C.Agardh) and Anti-tumor Activity,TS254.9
  21. Study on Preparation and Properties of Protein Isolates from Tilapia,TS254.4

CLC: > Agricultural Sciences > Aquaculture, fisheries > Aquatic basic science > Aquatic Biology > Aquatic Zoology
© 2012 www.DissertationTopic.Net  Mobile