|
Eggplant (Solanum melongena) originated in the tropical regions of India and Asia , Southeast Asia , ancient India as the earliest domesticated ground . China is the origin of the eggplant second . In this experiment, 16 eggplant strains for the material , optimized RAPD-PCR reaction system of eggplant , 200 pairs of primers were screened , and Materials cluster analysis . The results are as follows: 1. Established eggplant optimization of RAPD-PCR reaction system . Amplification total reaction volume of 10μL, containing 25ng template DNA , 1μL 10 × buffer , MgCl 2 3mmol L -1 , mM dNTP 200μmol · L -1 , primer concentration of 0.6 μmol · L -1 , 0.5 U Taq enzyme . PCR amplification reaction program : 94 ° C for 3 min ; 94 ° C for 30s, 39 ° C 30s, 72 ° C , 90s, 45 cycles ; 72 ° C for 7min . 2 . RAPD technique to analyze the genetic diversity of 16 copies of eggplant germplasm resources . 57 primers screened from 200 random primers , 551 bands were amplified polymorphic bands of 274 ( 49.73% ) ; primers amplified at least three with amplified up to 16 with an average of 9.67 each primer RAPD fragment . Of 3 software NTSYSpc RAPD data , the results showed that 16 Material genetic similarity coefficient ranged from 0.35 to 1.00 . The genetic similarity coefficient of 0.83 , the cluster analysis results can be tested materials are divided into three groups .
|