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Study on Seed Vigor of Wheat and Relationship between Seed Vigor and Enzymes Activation, Storage Protein in Wheat

Author: JiangWen
Tutor: YaoDaNian；ZhangWenMing
School: Anhui Agricultural University
Course: Crop Genetics and Breeding
Keywords: Wheat Seed vigor Enzyme activity Gliadin Glutenin
CLC: S512.1
Type: Master's thesis
Year: 2006
Downloads: 288
Quote: 4
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Abstract

This selection of 10 promotion in the production of wheat varieties: farmers 19 Annong 92484, WanXi 8906, Wanmai 19, Yumai 18, Yanzhan 4110, within Township 188 of Yang wheat 12, Huaimai, 20, Zhengmai9023, for the material. Five common indoor activity assay by studying the new seed and Seeds indicators Field Survival rate relationship, more accurate and effective method of determination of wheat seed vigor. On this basis, the study of new wheat seed Chen hydrogen peroxide enzyme (CAT), peroxidase (POD), superoxide dismutase (SOD) and dehydrogenase activity to explore the activity of seeds of wheat seeds The dynamic relationship. Further study of seed germination gliadin and gluten content of the process of change and the rate of degradation, explore a new method for determining the physiological and biochemical mechanism of wheat seed vigor and vitality. 1.5 different activity assay method measured wheat seeds (seeds and Seeds) vitality trend is not entirely consistent with the results of some indicators that reflect the level of vitality quite different. 2. The relationship between the conductivity and field seedling rate and other indicators are not significant. Seedling growth test, accelerated aging test, the simulated field emergence test seed viability test results seedling rate was significant or very significant positive correlation with the field, anti-cold determination of the rate of emergence and Field Survival rate was a significant positive correlation. According to the test results, that in addition to the conductivity outside, the other four kinds of determination methods can be used as an effective method for detection of wheat seed vigor. 3. Hydrogen peroxide enzyme (CAT), peroxidase (POD) and dehydrogenase activity greater differences between varieties, Wheat Seeds enzyme activity is lower than the new seed. Dehydrogenase activity and vitality index showed a significant positive correlation can be used as one of the indicators to assess seed vigor. 4. Different varieties of wheat seed size and seed storage protein content showed a significant positive correlation, seed size and storage protein content was negatively correlated with seed vigor, but not significantly. 5. Different levels of vitality wheat seed germination process, the dynamic changes of the storage protein content and degradation rate performance: high vigor seeds Yannong 19, gliadin content changes, degradation and rapid degradation of the protein content of the new seed 95.32 7d %, seed Chen degradation 88.94%; the low vigor the seed Zhengmai 9023 gliadin content and degradation slow to Section 7d new Gliadin degradation 67.39%, the degradation of seed Chen only 55.47%. The high vigor seeds Yannong 19 and the Valley low vigor seeds Zhengmai9023's protein content in the germination of the first 3d change more obvious little thereafter to 7d changes. According to the test results, it is possible to speculate that different vigor levels of different genotypes of wheat seed germination process, high vigor seed gliadin use efficiency was significantly higher than the low-vigor seeds, new seeds of the same species, is also higher than the old seeds. 6. SDS-PAGE electrophoresis pattern of wheat seed vigor levels reflect obvious A-PAGE electrophoresis pattern to reflect the vitality of wheat seeds, mainly in Wheat Seed Germination the first 3d molecular weight gliadin subunit bands how much 7d bands difference. High vigor seed Yannong 19 in the process of germination and growth, gliadin larger molecular weight proteins in imbibed 3d has started to degrade the first 7d completely degraded. While the weak vitality Zhengmai 9023 seeds in imbibed at 7d, bands are still clearly visible. According to the test results, it is possible to speculate that different levels of vitality wheat seed germination of the increase of the number of days, the high vigor seed gliadin molecular weight protein degradation significantly faster than low-vigor seeds, new seeds of the same variety is also faster than Seeds.

Study on Seed Vigor of Wheat and Relationship between Seed Vigor and Enzymes Activation, Storage Protein in Wheat

Abstract

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