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Study on Optimize Fermentation Conditions for Producing Podophyllotoxin from Endophytic Fungui

Author: CaoLi
Tutor: HuangJianXin
School: Northwestern University
Course: Microbiology
Keywords: Podophyllotoxin Podophyllotoxin glycosides Endophytic fungi Ferment Extraction
CLC: S567.3
Type: Master's thesis
Year: 2007
Downloads: 120
Quote: 2
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Abstract


Podophyllotoxin substances have significant anti-viral, anti-tumor activity in the field of medicine has important application value. Obtaining of such substances extracted from plants, but the limited resources of the natural plant, can not meet the market demand, endophytic fungi can produce and host the same metabolite features obtained by microbial fermentation of such substances, to get the ghost mortar toxin raw drug material resources to provide a new way. The current research in this area focused on the screening of valuable strains. In this study, obtained generate the material strains Md PODOPHYLLIN fermentation conditions to provide a reference for the active ingredient in the production of medicinal plants by microbial fermentation. Objective: to produce the podophyllotoxin substances endogenous fungal fermentation studies provide a new way, to solve PODOPHYLLIN anticancer drugs shortage; obtained by studying the active ingredient in the production of medicinal plants using microbial fermentation process data for the realization of the foundation of industrial production. Methods: thin layer chromatography, TLC scanning, HPLC, hydrogen nuclear magnetic resonance to determine the strain MD, Iy fermentation was the target component; design adapted to strain the Md, IY growth of semi-synthetic fermentation medium and optimized shake flask fermentation conditions. L 9 (3 4 ) orthogonal test the the fermentation cultures from strains Md extract PODOPHYLLIN substances extracted conditions. Results: determining strain Md can produce podophyllotoxin, the strain can produce podophyllotoxin glucoside Iy; an optimized semi-synthetic fermentation medium formula is: 2% starch, 2% sucrose, 0.2% peptone, 0.2% ammonium sulfate, 0.1% yeast extract, 0.02% K 2 HPO 4 0.02% the CaCO 3 , 0.02% NaCl, 100ml of water, pH 6.8. The fermentation medium, Md strain biomass increased by about 34.7%, the podophyllotoxin production increased by about 13.9%. Md strains optimized fermentation condition of: initial pH6.0, the medium volume 100mL/250mL flask, inoculum size 15%, 28 ° C and cultured for 7 days; flask fermentation conditions optimized strains Iy: Initial the pH6.5, medium volume 80mL/250mL flask, inoculum size 10%, 28 ° C for 6 days. Strains Md 50L fermenter amplification tests showed better cell growth, stable product synthesis. Determine the product downstream of the extraction, separation methods: mycelium frozen 4h, tissue mash crusher 20S, disrupted cell solution is extracted with 3 times the amount of chloroform three times, each time 10H; significantly improve the extraction rate. Conclusion: get podophyllotoxin class material obtained by fermentation and extracted from the fermentation product the substances PODOPHYLLIN technology roadmap, the research has the potential to achieve industrial production.

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CLC: > Agricultural Sciences > Crop > Economic crops > Medicinal crops > Fungus
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