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Genetic Diversity Analysis of Beauveria Bassiana by SSR and ISSR Technique

Author: Li
Tutor: FanMeiZhen；HuangYongPing
School: Anhui Agricultural University
Course: Microbiology
Keywords: Bassiana Molecular markers Genetic polymorphism Genetic differentiation
CLC: Q933
Type: Master's thesis
Year: 2006
Downloads: 201
Quote: 3
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Abstract


In this paper, SSR (microsatellite) markers and ISSR (simple sequence repeat spacer) mark, the four regions Dabie Langyashan collected 122 bassiana strains in Xuancheng, Guniujiang the genetic diversity analysis to explore the white and each region within the genetic diversity between the different regions of anisopliae obtained the following results:. material Screening: respectively, 15 pairs of SSR primers material and 20 ISSR primers material carried out the screening, to obtain the 13 pairs of micro-satellite primers material and 15 ISSR primers, these primers for PCR amplification of DNA bands can get a clear, high polymorphism. SSR and ISSR reaction system were established in each reaction system, can get a clear map. ISSR experiments 15 ISSR primers were amplified 244 with 97.95%, the percentage of polymorphic loci (PPL). The total Nei's genetic diversity index (H) .1659, the Shannon's information diversity index (I) was 0.2732. Population genetic diversity (Hs) 0.1697, the total gene diversity (Ht) was 0.1836. Between the various populations genetic differentiation coefficient (Gst) is 0.0757. Cluster analysis of genetic similarity between groups, Xuancheng and Guniujiang the genetic identity of up to 0.9962, the lowest genetic identity Xuancheng and Langyashan, 0.9600. From the Shannon index, the largest degree of variation in the population is Dabie Mountain area. SSR experiments, 13 pairs of SSR primers were amplified 293 with 98.98%, the percentage of polymorphic loci (PPL). The overall Nei's genetic diversity index (H) of 0.0408, the Shannon's information diversity index (I) was 0.0891. Population genetic diversity (Hs) 0.0391, the total gene diversity (Ht) was 0.0407. Between the various populations genetic differentiation coefficient (Gst) is 0.0384. Cluster analysis of genetic similarity between groups, Xuancheng and Guniujiang the genetic identity of up to 0.9990, the lowest genetic identity Xuancheng and Langyashan, 0.9965. From the Shannon index, the largest degree of variation in the population is Dabie Mountain area. ISSR analysis before the results are consistent. 5 by SSR and ISSR calculated the correlation coefficient between the two sets of similarity coefficient r = 0.9889 gt; r0.01 reached a very significant level, indicating that these two technologies SSR markers and ISSR analysis of genetic diversity bassiana a high level of consistency and credibility. The above results show that: the Anhui region bassiana high genetic diversity and genetic diversity of different populations there are some differences. Genetic differentiation coefficient that small genetic variation within populations showed a high level of genetic differentiation among populations.

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