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This paper is of entomogenous fungi Research Institute on the basis of Anhui Agricultural University for further study, the extension of Beauveria bassiana oil storage period. High quality spores from Beauveria bassiana Preparation and storage of oil before and after preparation, trying to extend the storage period of the room temperature bassiana oil processing and oil and mixed pesticide determination of compatibility departure explore some new ways. Test the main avenues of research and results are as follows: 1. Test from bassiana angle of the nutritional requirements of to add different carbon bassiana sporulation medium, nitrogen sources on the three strains (Bb13, Bb380, Bb202) were cultured and prepared spore powder, testing its spore-containing amount of germination rate, moisture content. By analyzing and comparing the filters out of the carbon prepared under the experimental conditions yield high powder high quality, strong vitality, low water content bassiana spores powder sporulation medium may be added to the top of sucrose, glucose, etc., the best The nitrogen source, nitrate, peptone, etc.. In addition, different bassiana strains of high temperature, lack of nutrition and other adverse environmental endurance, the experiment three different host strains produced spore powder quality testing found that strain BB13 spore powder production quality is superior to the other two strains. 2. Filter carbon and nitrogen sources on the basis of the test will bassiana spore powder preparation process required nutritional needs and culture conditions into account, added to the different incubation temperature, pH, and medium carbon nitrogen source, trace elements orthogonal experiments, the spore-containing spore germination rate of the main indicators, screening identified for Beauveria bassiana the BB13 spores grow best carbon source is glucose, nitrogen source peptone, trace elements copper and zinc can obviously promote sporulation and spore germination, the optimum pH value of 6 to 7, the optimal incubation temperature is 20 ° C to 25 ° C. 3. The spore powder during storage, temperature and water content of spores storage life. At 4 ° C and 25 ° C for a period of 18 months five water content of the spore powder the live spores determination, analysis of the results obtained, stored at 4 ℃, the water content of 1.25 to 9.87% processing spores 18 months of basic living spores rate maintained at more than 90%, the high water content processing (13.89%) spore powder slow decline in the first nine months of live spores from 97.38% to 77.94%, after living spores rate of decline accelerated to 420 days, all the spores inactivation, indicating that the high water content of the spore powder even if it is not cold storage for seven months (live spore was 84.11%); stored at 25 ° C under the spore powder of the water content of 13.89% in the first 30 days detects all the spores inactivation (not detected germination of spores); moisture content of 9.87%, 7.36% and 4.28% processing all the spores inactivation occurred at 195 days, 225 days and 420 days; minimum water content processing (1.25%) live spore rate of decline is apparently slow processing at a higher moisture content, the live spores rate in the 18 months, there was (15.94 ± 1.26)%. Thus, in spores stored at room temperature, the moisture content must be controlled at 5% or less, to a storage period of about 5 months and the live spore rate reached 80%. 4. Bassiana spores to limit nutrient culture, and high-temperature water bath adversity and formulated oil, oil dubbed Activity limit nutrition resulting spore spore rate compared with the control, both the live Alternaria rate of existence of a significant difference. Optimized medium with spores was prepared oil during storage, the spores the Activity spore rate over time gradually decreased, but the live spores rate of the measured value is higher than the corresponding period limit nutrition oil live spore. From 120 days, the limit carbon oil the live spores rate (38.3%) is significantly lower than the nitrogen-limited oil live spore rate (40.3%), and subsequently has been low, and there is significant difference to 240 days limit carbon finish live spore rate to 0, and the nitrogen-limited oil storage to 330 days, the live spore rate is maintained at 5.64%, which can be seen, to limit nitrogen cultured bassiana spores with oil storage period than limit the length of the carbon. Dubbed oil spores after a 50 ° C water bath processing, storage 270 days ago, spores in the oil the live spores rate compared with the control, are lower than that of the control, there is a significant difference, and the longer the water bath, spores live spore The rate is lower. But over time, the water bath spores oil live spore rate is lower than in the control decreased slowly to 300 days, spores oil live in the water bath after spore rate but higher than that of the control slowly, there are significant differences, so you can draw the appropriate bassiana spores high temperature processing, live spore late in the oil storage. In addition, the water bath for 30 minutes spores and water bath for 60 minutes spores oil after 300 days of storage, live spore rate there are significant differences water bath time is appropriate to extend the oil in the late spores can be extended in a certain extent, so it can be seen live spores rate. 5. Different time adding exogenous nutrition bassiana oil, compared with the control, the spore germination rate are improved, and basically there are significant difference with the control (germination rate of 5.42%). Respectively, the first three months of oil adding exogenous nutrition spore germination rate than three months after the effect of addition of better, and there are significant differences between them, especially in the first three months of added exogenous nutrition, The spores live spore rate of 8.41%, significantly higher than the control and three months after the addition of exogenous nutrition oil spores live spore rate, 6.03%, 5.59%, 5.74%, respectively. Bassiana oil stored at room temperature, the addition of exogenous nutrition in a timely manner, there is a certain effect live spore spore restore vitality, improve oil. 6. Beauveria bassiana spores good compatibility with salad oil, paraffin oil, but seen through the determination of heat resistance, the treatment temperature is low, the processing time length, different formulations bassiana conidia germination rate has an impact and viability. Agent and paraffin oil conidia as the treatment temperature increases, the processing time longer, the germination rate decreases; conidia agent for salad oil, in addition to their germination rate of more than 90% rate than the control has been postponed outside (about 3 hours), and did not affect their lives. Therefore, salad oil can be used as bassiana oil good oil carrier. 7. Bassiana determination of compatibility with dozens of pesticides: test ten selected pesticides with Beauveria bassiana spores compatibility order of: the Bacillus thuringiensis hydrogen bromide permethrin> dendrolimi virus> imidacloprid> triazole phosphorus> Bisultap> omethoate> avermectin> stuck grams> chlorfluazuron. Different concentrations of the same pesticide, the impact of pesticides on Beauveria bassiana spore germination and its concentration was significantly related, and bassiana conidia germination rate than the commonly used concentration (1.0 ×) dilution (0.1 × 0.2 × ) The following must be low. 8000IU Bacillus thuringiensis WP was diluted 5 times and 10 times the normal use concentration, 2.5% hydrogen bromide permethrin EC, Dendrolimus virus WP, 10% imidacloprid WP and 10-fold dilution of 2% triazophos EC, diluted 5-fold in 18% spore germination in Bisultap with the control was no significant difference, you can mix and bassiana oil, making it complementary synergism.
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